Skip to main content
SpringerLink
Log in

Lectin affinity chromatography

  • Protocol
  • Published:
Molecular Biotechnology Aims and scope Submit manuscript

Abstract

A protocol is described for the preparation of lectin affinity chromatography columns using purified lectins and preactivated matrices. A general method is given for the purification of glycoproteins on immobilized Con A. Methods for immobilizing Con A on CDI agarose, Affi-Gel 15, and carbonyl-diimidazole-activated agarose are described.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kennedy, J. F. and Rosevear, A. (1973) An assessment of the fractionation of carbohydrates on Concanavalin A Sepharose by affinity chromatography.J. Chem. Soc. Perkin Trans. 19, 2041–2046.

    Article  CAS  Google Scholar 

  2. Fidler, M. B., Ben-Yoseph, Y., and Nadler, H. L. (1979) Binding of human liver hydrolases by immobilised lectins.Biochem. J. 177, 175.

    Google Scholar 

  3. Kawai, Y. and Spiro, R. G. (1980) Isolation of major glycoprotein from fat cell plasma membranes.Arch. Biochem. Biophys. 199, 84–91.

    Article  PubMed  CAS  Google Scholar 

  4. Sharma, S. K. and Mahendroo, P. P. (1980) Affinity chromatography of cells and cell membranes.J. Chromatography 184, 471–499.

    Article  CAS  Google Scholar 

  5. Cummings, R. D. and Kornfeld, S. (1982) Fractionation of asparagine linked oligosaccharides by serial lectin affinity chromatography.J. Biol. Chem. 257, 11,235–11,240.

    CAS  Google Scholar 

  6. Boyle, F. A. and Peters, T. J. (1988) Characterisation of galactosyltransferase isoforms by ion exchange and lectin affinity chromatography.Clin. Chim. Acta 178, 289–296.

    Article  PubMed  CAS  Google Scholar 

  7. Pos, O., Drechoe, A., Durand, G., Bierhuizen, M. F. A., Van der Stelt, M. E., and Van Dik, W. (1989) Con A affinity of rat α-1-acid glycoprotein (rAGP); Changes during inflammation, dexamethasone or phenobarbital immuno-electrophoresis(CAIE) are not only a reflection of biantennary glycan content.Clin. Chim. Acta 184, 121–132.

    Article  PubMed  CAS  Google Scholar 

  8. Debray, H., Pierce-Cretel, A., Spik, G., and Montreuil, J. (1983) Affinity of ten lectins towards various glycopeptides with the N-glycosylamine linkage and related oligosaccharides, inLectins in Biology, Biochemistry, Clinical Biochemistry, (Bog-Hanson, T. C. and Spengler, G. A., eds.) De Gruyter, Berlin, vol. 3, p. 335.

    Google Scholar 

  9. Debray, H., Descout, D., Strecker, G., Spik, G., and Montreuil, J. (1981) Specificity of twelve lectins towards oligosaccharides and glycopeptides related toN-glycosylglycoproteins.Eur. J. Biochem. 117, 41–55.

    Article  PubMed  CAS  Google Scholar 

  10. Nikawa, T., Towatari, T., and Katunuma, N. (1992) Purification and characterisation of Cathepsin J from rat liver.Eur. J. Biochem. 204, 381–393.

    Article  PubMed  CAS  Google Scholar 

  11. Esmann, M. (1980) Concanavalin A Sepharose purification of soluble Na,K-AT-Pase from rectal glands of the spiny dogfish,Anal. Biochem. 108, 83–85.

    Article  PubMed  CAS  Google Scholar 

  12. Weinstein, Y., Givol, D., and Strausbauch, D. (1972) The fractionation of immunoglobulins with insolubilised concanavalin A.J. Immunol. 109, 1402–1404.

    PubMed  CAS  Google Scholar 

  13. Yamaguchi, N., Kawai, K., and Ashihara, T. (1986) Discrimination of gamma-glutamyltranspeptidase from normal and carcinomatous pancreas.Clin. Chim. Acta 154, 133–140.

    Article  PubMed  CAS  Google Scholar 

  14. Matsuura, S. and Chen, H. C. (1980) Simple and effective solvent system for elution of gonadotropins from concanavalin A affinity chromatography.Anal. Biochem. 106, 402–410.

    Article  PubMed  CAS  Google Scholar 

  15. Kinzel, V., Kübler, D., Richards, J., and Stöhr, M. (1976)Lens Culinaris lectin immobilised on sepharose: Binding and sugar specific release of intact tissue culture cells.Science 192, 487–489.

    Article  PubMed  CAS  Google Scholar 

  16. Kvist, S., Sandberg-Trägardh, L., and Östberg, L. (1977) Isolation and partial characterisation of papain solubilised murine H-2 antigens.Biochemistry 16, 4415–4420.

    Article  PubMed  CAS  Google Scholar 

  17. Dawson, J. R., Silver, J., Shepherd, L. B., and Amos, B. D. (1974) The purification of detergent solubilised HL-A antigen by affinity chromatography with the haemagglutin fromLens culinaris.J. Immunol. 112, 1190–1193.

    PubMed  CAS  Google Scholar 

  18. Pujol, F. H. and Cesari, I. M. (1993) Schistosoma mansoni: surface membrane isolation with lectin coated beads.Membr. Biochem. 10, 155–161.

    Article  PubMed  CAS  Google Scholar 

  19. Gougos, A. and Letarte, M. (1988) Biochemical characterisation of the 44g4 antigen from the HOON preB leukemic cell line.J. Immunol. 141(6), 1934–1940.

    PubMed  CAS  Google Scholar 

  20. Jackson, S. F. and Tjian, R. (1989) Purification and analysis of RNA polymerase 11 transcription factors by using wheat germ agglutinin affinity chromatography. P. N. A. S.86(6), 1781–1785.

    Article  PubMed  CAS  Google Scholar 

  21. Dulaney, J. T. (1979) Factors affecting the binding of glycoproteins to concanavalin A andRicinus communis agglutin and the dissociation of their complexes.Anal. Biochem. 99, 254.

    Article  PubMed  CAS  Google Scholar 

  22. Gregory, R. L., Rundergren, J., and Arnold, R. R. (1987) Separation of human IgA1 and IgA2 using jacalin-agarose chromatography.J. Immunol. Methods 99, 101–106.

    Article  PubMed  CAS  Google Scholar 

  23. Pilatte, Y., Hammer, C. H., and Frank, M. M. (1983) A new simplified procedure for Cl inhibitor purification.J. Immunol. Methods 120, 37–43.

    Article  Google Scholar 

  24. Blake, D. A. and Goldstein, I. J. (1982) Resolution of carbohydrates by lectin affinity chromatography.Methods Enzymol. 83, 127–132.

    PubMed  CAS  Google Scholar 

  25. Matson, R. S. and Siebert, C. J. (1988) Evaluation of a new N-hydroxysuccinimide activated support for fast flow immunoaffinity chromatography.Prep. Chromatogr. 1, 67–91.

    Article  Google Scholar 

  26. Lotan, R. and Nicolson, G. L. (1979) Purification of cell membrane glycoproteins by lectin affinity chromatography.Biochem. Biophys. Acta 559, 329–376.

    PubMed  CAS  Google Scholar 

  27. Cook, J. H. (1984) Turnover and orientation of the major neural retina cell surface protein protected from tryptic cleavage.Biochemistry 23, 899–904.

    Article  PubMed  CAS  Google Scholar 

  28. Mintz, G. and Glaser, L. (1979) Glycoprotein purification on a high capacity wheat germ lectin affinity column.Anal. Biochem. 97, 423–427.

    Article  PubMed  CAS  Google Scholar 

  29. Roque-Barreira, M. C. and Campos-Neto, A. (1985) Jacalin: An IgG-binding lectin.J. Immunol. 134, 1740.

    PubMed  CAS  Google Scholar 

  30. Lotan, R., Beattie, G., Hubbell, W., and Nicolson, G. L. (1977) Activities of lectins and their immobilised derivatives in detergent solutions. Implications on the use of lectin affinity chromatography for the purification of membrane glycoproteins.Biochemistry 16(9), 1787.

    Article  PubMed  CAS  Google Scholar 

  31. Narasimhan, S., Wilson, J. R., Martin, E., and Schacter, H. (1979) A structural basis for four distinct elution profiles on concanavalin A Sepharose affinity chromatography of glycopeptides.Can. J. Biochem. 57, 83–97.

    Article  PubMed  CAS  Google Scholar 

  32. Poliquin, L. and Shore, G. C. (1980) A method for efficient and selective recovery of membrane glycoproteins from concanavalin A Sepharose using media containing sodium dodecyl sulphate and urea.Anal. Biochem. 109, 460–465.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. “White Hayes” Garden Close, Givons Grove, KT22 8LT, Leatherhead, Surrey, UK

    Iris West & Owen Goldring

Authors
  1. Iris West
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Owen Goldring
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

West, I., Goldring, O. Lectin affinity chromatography. Mol Biotechnol 2, 147–155 (1994). https://doi.org/10.1007/BF02824807

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02824807

Index Entries

Search

Navigation