Abstract
Libraries of plasmid clones covering the entire chloroplast (cp) genome of the common wheat,Triticum aestivum cv. Chinese Spring were constructed and assembled into contig-clones. From these, we obtained the complete nucleotide sequence of wheat chloroplast DNA—a 134,540 bp circular DNA (DDBJ accession no. AB042240) containing four species of ribosomal RNA, 30 genes for 20 species of transfer RNA, and 71 protein coding genes. Additionally, we detected five unidentified open reading frames conserved among grasses. Plasmid clones are available on request.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Hallick RB, Hong L, Drager RG, Favreau MR, Monfort A, Orsat B, Spielmann A and Stutz E (1993) Complete sequence ofEuglena gracilis chloroplast DNA. Nucleic Acids Res 21: 3537–3544.
Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Honji Y, Sun CR, Meng BY, Li YQ, Kanno A, Nishizawa Y, Hirai A, Shinozaki K and Sugiura M (1989) The complete sequence of the rice (Oryza sativa) chloroplast genome: Intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals. Mol Gen Genet 217: 185–194.
Howe CJ, Barker RF, Bowman CM and Dyer TA (1988) Common features of three inversions in wheat chloroplast DNA. Curr Genet 13: 343–349.
Maier RM, Neckermann K, Igloi GL and Koessel H (1995) Complete sequence of the maize chloroplast genome: Gene content, hotspots of divergence and fine tuning of genetic information by transcript editing. J Mol Biol 251: 614–628.
Ogihara Y and Tsunewaki K (1982) Molecular basis of the genetic diversity of the cytoplasm inTriticum andAegilops: I. Diversity of the chloroplast genome and its lineage revealed by the restriction pattern of ct-DNAs. Jpn J Genet 57: 371–396.
Ogihara Y and Tsunewaki K (1988) Diversity and evolution of chloroplast DNA inTriticum andAegilops as revealed by restriction fragment analysis. Theor Appl Genet 76: 321–332.
Ogihara Y, Terachi T and Sasakuma T (1988) Intramolecular recombination of chloroplast genome mediated by short direct-repeat sequences in wheat species. Proc Natl Acad Sci USA 85: 8573–8577.
Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H and Ozeki H (1986) Chloroplast gene organization deduced from complete sequence of liverwortMarchantia polymorpha chloroplast DNA. Nature 322: 572–574.
Palmer J (1991) Plastid chromosomes: structure and evolution. In: Bogorad L and Vasil IK (eds), The Molecular Biology of Plastids, pp 5–53, Academic Press, San Diego.
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Simada H and Sugiura M (1986) The complete nucleotide sequence of the tobacco chloroplast genome: Its gene organization and expression. EMBO J 5: 2043–2049.
Wakasugi T, Tsudzuki J, Ito S, Nakashima K, Tsudzuki T and Sugiura M (1994) Loss of all ndh genes as determined by sequencing the entire chloroplast genome of the black pinePinus thunbergii. Proc Natl Acad Sci USA 91: 9794–9798.
Wakasugi T, Nagai T, Kapoor M, Sugita M, Ito M, Ito S, Tsudzuki J, Nakashima K, Tsuzuki T, Suzuki Y, Hamada A, Ohta T, Inamura A, Yoshinaga K and Sugiura M (1997) Complete nucleotide sequence of the chloroplast genome from the green algaChlorella vulgaris: The existence of genes possibly involved in chloroplast division. Proc Natl Acad Sci USA 94: 5967–5972.
Wakasugi T, Sugita M, Tsudzuki T and Sugiura M (1998) Updated gene map of tobacco chloroplast DNA. Plant Mol Biol Reptr 16: 231–241.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ogihara, Y., Isono, K., Kojima, T. et al. Chinese spring wheat (Triticum aestivum L.) chloroplast genome: Complete sequence and contig clones. Plant Mol Biol Rep 18, 243–253 (2000). https://doi.org/10.1007/BF02823995
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02823995