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Effect of medium on the bacteriocin production byKlebsiella pneumoniae

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Abstract

The influence of growth media and media constituents on bacteriocin production byKlebsiella pneumoniae was studied. Among the standard laboratory media used trypticase soy broth (TSB) showed the maximum production and poor yields resulted from growth in peptone water and nutrient broth. A number of peptones differed in their bacteriocin production. Best yields were observed in tryptone and proteose peptone water. Addition of 1 % yeast extract to TSB further stimulated bacteriocin production. However, activity was low when glucose, glycine, sodium mercaptoacetate or bile salt mixture were added to the medium. Supression of synthesis by certain agents as well as inhibition of formed bacteriocin by the others appears to affect the bacteriocin yield. No proteinase activity was detected during the entire incubation period.

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References

  • Bauernfeind A.: Epidemiological typing of klebsiella by bacteriocins, pp. 213–224 inMethods in Microbiology, Vol. 16 (ed. T. Bergan). Academic Press, New York 1984.

    Google Scholar 

  • Buffenmeyer C.L., Rycheck R.R., Yee R.B.: Bacteriocin (klebocin) sensitivity typing of klebsiella.J. Clin. Microbiol. 4, 239–244 (1976).

    Google Scholar 

  • Chhibber S.: Physico-chemical and immunological properties of klebocin.PhD Thesis, Panjab University, Chandigarh (India) 1984.

    Google Scholar 

  • Chhibber S., Vadehra D.V.: Purification and characterization of a bacteriocin fromKlebsiella pneumoniae 158.J. Gen. Microbiol. 132, 1051–1054 (1986).

    PubMed  CAS  Google Scholar 

  • Chhibber S., Goel A., Kapoor N., Saxena M., Vadehra D.V.: Bacteriocin (klebocin) typing of clinical isolates ofKlebsiella pneumoniae.Eur. J. Epidemiol. 4, 115–118 (1988).

    Article  PubMed  CAS  Google Scholar 

  • Chugh T.D., Walia S.K., Sabharwal U.: Bacteriocin (klebocin) sensitivity typing as an epidemiological marker ofKlebsiella pneumoniae.Ind. J. Med. Res. 71,195–202 (1980).

    CAS  Google Scholar 

  • Chugh T.D., Sabharwal U., Arora D.R., Kaur H., Sikka R., Walia S.K.: Klebocin typing—a national level survey.Ind. J. Med. Res. 75, 791–795 (1982).

    CAS  Google Scholar 

  • Durkacz B.W., Kennedy C.K., Sherratt D.J.: Plasmid replication and the induced synthesis of colicins E1 and E2 inEscherichia coli.J. Bacteriol. 117, 940–946 (1974).

    PubMed  CAS  Google Scholar 

  • Isaacson R.E., Konisky J.: Studies on the regulation of colicin Ib synthesis. II. The synthesis of ColIb specific RNAin vivo.Mol. Gen. Genet. 132, 223–232 (1974).

    Article  PubMed  CAS  Google Scholar 

  • Israil A.M.: Studies concerning the sensitivity ofKlebsiella strains to a set ofKlebsiella bacteriocins.Zbl. Bakteriol. Hyg. I. Abt. Orig. A 248, 91–98 (1980).

    CAS  Google Scholar 

  • Israil A.M.: The significance of bacteriocin typing ofKlebsiella strains.Zbl. Bakteriol. Hyg. I. Abt. Orig. A 250, 63–71 (1981).

    CAS  Google Scholar 

  • Kaur I., Prakash K., Sharma K.B.: Klebocin types in urinary and purulent fluids isolates ofKlebsiella pneumoniae.Ind. J. Med. Res. 77, 599–601 (1983).

    CAS  Google Scholar 

  • Keay L., Wildi B.S.: Protease of genuesBacillus. I. Neutral proteases.Biotechnol. Bioeng. 12, 179–182 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Kusek J.W., Herman L.G.: Typing ofProteus mirabilis by bacteriocin production and sensitivity as a possible epidemiological marker.J. Clin. Microbiol. 12, 112–120 (1980).

    PubMed  CAS  Google Scholar 

  • Miller J.H.,Experiment in Molecular Genetics. Cold Spring Harbor, New York. Cold Spring Harbor Laboratory 1972.

    Google Scholar 

  • Nakazawa, Tamada T.: Stimulation of colicin EI synthesis by cyclic 3′,5′-adenosine monophosphate in mitomycin C inducedEscherichia coli.Biochem. Biophys. Res. Comm. 46, 1004–1010 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Rogers A.H.: Effect of the medium on bacteriocin production among strains ofStreptococcus mutans.Appl. Microbiol. 24, 294–295 (1972).

    PubMed  CAS  Google Scholar 

  • Van Tiel-Menkveld G.J., Rezea A., De Graaf F.K.: Production and excretion of cloacin DF13 byEscherichia coli harboring plasmid Clo DF 13.J. Bacteriol. 140, 415–423 (1979).

    Google Scholar 

  • Walia V., Saigal R.K., Kanta S., Kaur A.: Klebocin typing ofKlebsiella pneumoniae.Ind. J. Microbiol. 23, (2), 65–69 (1983).

    Google Scholar 

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  1. D. V. Vadehra

    Present address: Westreco Inc., Boardman Road, 06776, New Milford, Connecticut, USA

Authors and Affiliations

  1. Department of Microbiology, Panjab University, 160 014, Chandigarh, India

    S. Chhibber & D. V. Vadehra

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  1. S. Chhibber
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  2. D. V. Vadehra
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Chhibber, S., Vadehra, D.V. Effect of medium on the bacteriocin production byKlebsiella pneumoniae . Folia Microbiol 34, 99–105 (1989). https://doi.org/10.1007/BF02823686

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