Skip to main content
SpringerLink
Log in

Fructose-bisphosphatase-deficient mutants of mucoidPseudomonas aeruginosa

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Fructose-bisphosphatase-deficient mutants of mucoidPseudomonas aeruginosa were isolated by ethyl methanesulfonate mutagenesis using gluconate as the nonpermissive substrate, and all the sixty isolates possessed 10–30% of the parental enzyme activity. The mutants had low levels of fructose-bisphosphate aldolase activity and could not normally synthesize alginate from any substrate except onPseudomonas isolation agar plates. The results suggest the essentiality of fructose bisphosphatase activity for the growth or survival ofP. aeruginosa and a probable linkage of genes controlling this enzyme with those of fructose bisphosphate aldolase and alginate biosynthesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Banerjee P.C., Vanags R.I., Chakrabarty A.M., Maitra P.K.: Alginic acid synthesis inPseudomonas aeruginosa mutants defective in carbohydrate metabolism.J. Bacteriol. 155, 238 (1983).

    PubMed  CAS  Google Scholar 

  • Banerjee P.C., Vanags R.I., Chakrabarty A.M., Maitra P.K.: Fructose 1,6-bisphosphate aldolase activity is essential for synthesis of alginate from glucose byPseudomonas aeruginosa.J. Bacteriol. 161, 458 (1985).

    PubMed  CAS  Google Scholar 

  • Banerjee P.C., Darzins A., Maitra P.K.: Gluconeogenic mutations inPseudomonas aeruginosa: Genetic linkage between fructose bisphosphate aldolase and phosphoglycerate kinase.J. Gen. Microbiol. 133, 1099 (1987).

    PubMed  CAS  Google Scholar 

  • Brammer W.J., Clarke P.H.: Induction and repression ofPseudomonas aeruginosa amidase.J. Gen. Microbiol. 37, 307 (1964).

    Google Scholar 

  • Darzins A., Chakrabarty A.M.: Cloning of genes controlling alginate biosynthesis from a mucoid cystic fibrosis isolate ofPseudomonas aeruginosa.J. Bacteriol. 159, 9 (1984).

    PubMed  CAS  Google Scholar 

  • Darzins A., Wang S.K., Vanags R.I., Chakrabarty A.M.: Clustering of mutations affecting alginic acid biosynthesis in mucoidPseudomonas aeruginosa.J. Bacteriol. 164, 516 (1985).

    PubMed  CAS  Google Scholar 

  • Fraenkel D.G., Horecker B.L.: Fructose 1,6-diphosphatase and acid hexose phosphatase ofEscherichia coli.J. Bacteriol. 90, 837 (1965).

    PubMed  CAS  Google Scholar 

  • Fujita Y., Freese E.: Isolation and properties of aBacillus subtilis mutant unable to produce fructose bisphosphatase.J. Bacteriol. 145, 760 (1981).

    PubMed  CAS  Google Scholar 

  • Hochster R.M., Katznelson H.: On the mechanism of glucose-6-phosphate oxidation in cell-free extracts ofXanthomonas phaseoli.Can. J. Biochem. Physiol. 36, 669 (1958).

    PubMed  CAS  Google Scholar 

  • Lessie T.G., Phibbs P.V. Jr.: Alternative pathways of carbohydrate utilisation in pseudomonads.Ann. Rev. Microbiol. 38, 359 (1984).

    Article  CAS  Google Scholar 

  • Ohman D.E., Chakrabarty A.M.: Genetic mapping of chromosomal determinants for the production of the exopolysaccharide alginate in aPseudomonas aeruginosa cystic fibrosis isolate.Infect. Immun. 33, 142 (1981).

    PubMed  CAS  Google Scholar 

  • Wood H., O'Brien W.E., Michaels G.: Properties of carboxytransphosphorylase, pyruvate phosphate dikinase, pyrophosphate-phosphofructokinase and pyrophosphate-acetate kinase and their roles in the metabolism of inorganic pyrophosphate.Adv. Enzymol. 45, 85 (1977).

    PubMed  CAS  Google Scholar 

Download references

Author information

Author notes

  1. P. C. Banerjee

    Present address: Indian Institute of Chemical Biology, 700 032, Calcutta, India

Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Illinois, 60612, Chicago, Illinois, USA

    P. C. Banerjee

Authors
  1. P. C. Banerjee
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Banerjee, P.C. Fructose-bisphosphatase-deficient mutants of mucoidPseudomonas aeruginosa . Folia Microbiol 34, 81–86 (1989). https://doi.org/10.1007/BF02823683

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02823683

Keywords

Search

Navigation