Effect of strictly plant-related factors on the response ofHevea brasiliensis andTheobroma Cacao nodal explants culturedIn vitro

  • L. Lardet
  • M. E. Aguilar
  • N. Michaux-Ferrière
  • M. Berthouly
Developmenal Biology/Morphogenesis

Summary

Nodal cuttings (primary nodal explants) were used to initiate microcutting cultures ofHevea brasiliensis andTheobroma cacao. The physiological stage of growth reached by the branches from which the explants were taken had a considerable influence on culture initiation and response. In both species, the buds of the nodal explants taken from dormant branches (stages 11, 12 and 13 inTheobroma; stage D inHevea) revealed greater opening abilityin vitro and shoot elongation than buds taken from branches in a phase of active meristem growth inTheobroma (stages F1 and F2) or during a foliar growth phase inHevea (stage C). InHevea, the branch growth phase effect persisted into the multiplication phase when shoots developedin vitro were used as the source of secondary nodal explants. However, it disappeared during subcultures of primary nodal explants. In both species, the position of the nodes on the branch with respect to the apex also had an effect on culture initiation. The buds of nodes furthest from the apex were more likely to develop into shoots. InHevea, the position of secondary nodal explants obtained from the primary shoot was seen to vary in effect depending on growth regulator applications during primary culture. These results are analyzed in terms of variations in the balance between endogenous growth promoters/inhibitors in the buds, depending on morphogenic stages and the bud position on the branch.

Key words

rubber tree cocoa tree rhythmic growth microcuttings hormones 

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References

  1. Amin, M. N.; Jaiswal, V. S. Rapid clonal propagation of guava throughin vitro shoot proliferation on nodal explants of mature trees. Plant Cell tissue Organ Cult, 9:235–243; 1987.CrossRefGoogle Scholar
  2. Amin, M. N.; Jaiswal, V. S.In vitro response of apical bud explants from mature trees of jackfruit (Artocarpus heterophyllus) Plant Cell Tissue Organ Cult. 33:59–65; 1993.CrossRefGoogle Scholar
  3. Bertrand, B. Quelques aspects du microbouturage du cacaoyer (Theobroma cacao L.). Café Cacao Thé 31:175–182; 1987.Google Scholar
  4. Bonga, J. M. Organogenesisin vitro of tissues from mature conifers. In Vitro 17:511–518; 1981.CrossRefGoogle Scholar
  5. Boulay, M. Propagationin vitro du Douglas par micropropagation de germination aseptique et culture de bourgeons dormants. Etud. Rech. AF-OCEL 12:67–75; 1979.Google Scholar
  6. Brand, M. H.; Lineberger, R. D.In vitro propagation ofHalesia carolina L. and the influence of explantation timing on initial shoot proliferation. Plant Cell Tissue Organ Cult. 7:103–113; 1986.CrossRefGoogle Scholar
  7. Brand, M. H. Initiating cultures ofHalesia andMalus: influence of flushing stage and benzyladenine. Plant Cell Tissue Organ Cult. 33:129–132; 1993.CrossRefGoogle Scholar
  8. Carron, M. P.; Enjalric, F.; Lardet, L., et al. Rubber (Hevea brasiliensis Müll. Arg.). In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry. Vol. 5, Trees Il. Berlin Heidelberg: Springer-Verlag; 1989:221–245.Google Scholar
  9. Chèvre, A. M.; Gill, S. S.; Mouras, A., et al.In vitro vegetative multiplication of chesnut. J. Hort. Sci. 58:23–29; 1983.Google Scholar
  10. Dubois, P.. Contribution à l'étude de la croissance par poussées successives chezHevea brasiliensis Müll. Arg. Agricultura, Louvain 10:125–149; 1962.Google Scholar
  11. Dufour, M.; Dublin, P. Quelques données sur la multiplication végétativein vitro des cacaoyers cultivés (Theobroma cacao L.). Café Cacao Thé 29:235–244; 1985.Google Scholar
  12. Enjalric, F.; Carron, M. P. Microbouturage,in vitro de jeunes plantsd'Hevea brasiliensis (Kunth., Müll. Arg). C. R. Acad. Sci. Paris, t. 295, Série III:259–264; 1982.Google Scholar
  13. Flynn, W. P.; Glicenstein, L. J.; Fritz P. J.Theobroma cacao L.: An axillary budin vitro propagation procedure. Plant Cell Tissue Organ Cult. 20:111–117; 1990.CrossRefGoogle Scholar
  14. Gener, P. Le greffage de l'hévéa-Influence des stades de poussée foliaire du greffon et du porte-greffe sur la résussite au greffage Essai de mise en évidence de l'évolution de la concentration en AIA dans les jeunes bourgeons d'hévéa. Mémoire de fin d'étude, Ecole supérieure d'agriculture de Purpan, Toulouse, 1966: 51 p.Google Scholar
  15. Greathouse, D. C.; Laetsch, W. M.; Phinney, B. O. The shoot growth rhythm of tropical treeTheobroma cacao L. Am. J. Bot. 58:281–286; 1971.CrossRefGoogle Scholar
  16. Greenwood, D. C.; Posnette, A. F. The growth flushes of cocoa. J. Hort. Sci. 25:164–174; 1950.Google Scholar
  17. Hallé F.; Martin, R. Etude de la croissance rythmique chez l'Hévéa (Hevea brasiliensis Müll. Arg. Euphorbiacée, Crotonoîdées). Adansonia 8:475–504; 1968.Google Scholar
  18. Hutchinson, J. F.In vitro propagation of apple using organ plant tissue culture. 5th International Congress of Plant Tissue and Cell Culture: 729–730; 1982.Google Scholar
  19. Jonard, R.; Baud P.; Valderon, G. Développementin vitro de plantes à partir d'apex prélevés sur un cultivar de figuier (Ficus canica L.). Bull. Soc. Bot. Fr., 130, Lett. Bot. 415:301–306; 1983.Google Scholar
  20. Lardet, L.; Enjalric, F.; Carron, M. P. Culture d'apexd'Hevea brasiliensis (Müll. Arg.) Influence du stade morphogénétique et de l'âge des explants. C. R. Acad. Sci. Paris, t. 310, Série III:195–202; 1990.Google Scholar
  21. Meier, K.; Reuther, G. Factors controlling micropropagation of matureFagus sylvatica. Plant Cell Tissue Organ Cult. 39:231–238; 1994.CrossRefGoogle Scholar
  22. Meynier, V. Contribution de la culture de méristèmes à la micropropagationin vitro du noyer. Ann. Rech. sylvicoles AFOCEL: 75–85; 1984.Google Scholar
  23. Morel, G. Recherches sur la culture associée de parasites obligatoires de tissus végétaux. Ann. Euphyties 14:123–234; 1948.Google Scholar
  24. Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–479; 1962.CrossRefGoogle Scholar
  25. Nadel, B. L.; Altman, A.; Pleban, S.., et al.In vitro development of matureFagus sylvatica L. buds. II. Seasonal changes in the response to plant growth regulators. J. Plant Physiol. 138:136–141; 1991.Google Scholar
  26. Orchard, J. E.; Collin, H. A.; Hardwick, K. Culture of shoot apices ofTheobroma cacao. Physiol. Plant. 47:207–210; 1979.CrossRefGoogle Scholar
  27. Orchard, J. E.; Collin, H. A.; Hardwick, K. Biochemical and physiological aspects of leaf development in cocoa (Theobroma cacao) IV: changes in growth inhibitors. Plant Sci. Lett. 18:299–305; 1980.CrossRefGoogle Scholar
  28. Passey, A. J.; Jones, O. P. Shoot proliferation and rootingin vitro ofTheobroma cacao L. type Amelonado. J. Hort. Sci. 58:589–592; 1983.Google Scholar
  29. Quoirin, M.; Boxus, P.; Gaspar, T. Root initiation and isoperoxydase of stem tip cuttings from maturePrunus plants. Physiol. Vég. 12:165–174; 1974.Google Scholar
  30. Quoirin, M.; Gaspar, T.; Boxus, P. Substances de croissance endogènes liées à la reprise de méristèmes dePrunus accolade en culturein vitro. C. R. Acad. Sci. Paris, t 281:1309–1312; 1975.Google Scholar
  31. Selby, C.; Harvey, B. M. R. The influence of natural andin vitro bud flushing on adventitious bud production in sitka spruce (Picea sitchensis Bong.) bud and needle cultures. New Phytol. 100:549–562; 1985.CrossRefGoogle Scholar
  32. Sutter, E. G.; Barker, P. B.In vitro propagation of matureLiquidambar styraciflua. Plant Cell Tissue Organ Cult. 5:13–21; 1985.CrossRefGoogle Scholar
  33. Wright, S. T. C. Seasonal changes in the levels of free and bound abscisic acid in black currant (Ribes nigrum) and beech (Fagus sylvatica) buds. J. Exp. Bot. 26:161–174; 1975.CrossRefGoogle Scholar
  34. Zieslin, N.; Spielgelstein, H.; Halevy, A. H. Components of axillary bud inhibition in rose plants. IV. Inhibitory activity of plant extracts. Bot. Gaz. 139:64–68; 1978.CrossRefGoogle Scholar

Copyright information

© Society for In Vitro Biology 1998

Authors and Affiliations

  • L. Lardet
    • 1
  • M. E. Aguilar
    • 2
  • N. Michaux-Ferrière
    • 3
  • M. Berthouly
    • 4
  1. 1.Programme Hévéa, Unité BIOTROPCIRAD-CPMontpellier cedexFrance
  2. 2.Unité de biotechnologie, Centre Agronomique Tropical pour la Recherche et l'EnseignementCATIETurrialbaCosta Rica
  3. 3.Laboratoire d'Histo-Cytologie, Unité BIOTROPCIRAD-GERDATMontpellier cedexFrance
  4. 4.Programme Cacao, Unité BIOTROPCIRAD-CPMontpellier cedexFrance

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