Summary
Continuous culture is an attractive research tool in physiologic and growth and production kinetics research. However, fulfillment of the basic assumptions of continuous culture in the experimental set-up may cause problems. The homogeneity of plant cell cultures and effluent, particularly, may cause problems. This paper presents an experimental set-up which solves these problems and describes the use of this equipment in a study of the growth kinetics of plant cells. Industrial application of the continuous culture of plant cells in the production of secondary metabolites seems to be profitable when compared with batch or fed-batch cultures. However, various problems such as uncoupled product formation and strain instability make fed-batch culture a better choice.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bertola, M. A.; Roemer, R. G. M.; Klis, F. M. Growth and cell well composition of glucose-limited bean cells (Phaseolus vulgaris L.). In: Bertola, M. A., ed. Continuous culture of glucose-limited bean cells. The Netherlands: Univ Amsterdam; Thesis. 1980.
Calcott, P. H. Continuous cultures of cells. Boca Raton, FL: CRC Press; 1981.
Deus-Neumann, B.; Zenk, M. H. Instability of indole alkaloid production inCatharanthus roseus cell suspension cultures. Planta Med. 162:427–431; 1984.
Heijnen, J. J. Terwisscha van Scheltiwga, A. H.; Straathof, A. J. Fundamental bottlenecks in the application of continuous bioprocesses. J. Biotechnol. 22:3–20; 1992.
Kreis, W.; Reinhard, E. Production of deacetyllanatoside C byDigitalis lanata cell cultures. In: Nijkamp, H. J. J.; van der Plas, L. H. W.; van Aartrijk, J., eds. Progress in plant cellular and molecular biology. Dordrecht, The Netherlands: Kluwer Academic Publishers; 1990:706–711.
Kurz, W. G. W. A chemostat for growing higher plant cells in single cell suspension cultures. Exp. Cell Res. 64:476–479; 1971.
Meijer, J. J. Effects of hydrodynamic and chemical/osmotic stress on plant cells in a stirred bioreactor. The Netherlands: Delft Univ. Technology Thesis; 1989.
Miller, R. A.; Shyluk, J. P.; Gamborg, O. L., et al. Phytostat for continuous culture and automatic sampling of plant-cell suspensions. Science 159:540–542; 1968.
Noorman, H. J., Baksteen, J.; Heijnen, J. J., et al. The bioreactor overflow device: an undesired selective separator in continuous cultures? J. Gen. Microbiol. 137:2171–2177; 1991.
Sahai, O. P.; Shuler, M. L. On the nonideality of chemostat operation using plant cell suspension cultures. Can. J. Bot. 60:692–700; 1982.
Scragg, A. H.; Allan, E. J.; Leckie, F. Effect of shear on the viability of plant cell suspensions. Enzyme Microb. Technol. 10:361–367; 1988.
Tulecke, W. Continuous cultures of higher plant cells in liquid media: the advantages and potential use of a phytostat. Ann. NY Acad. Sci. 139:162–175; 1966.
Van Gulik, W. M.; Meijer, J. J.; ten Hoopen, H. J. G., et al. Growth of aCatharanthus roseus cell suspension culture in a modified chemostat under glucose limiting conditions. Appl. Microbiol. Biotechnol. 30:270–275; 1989.
Van Gulik, W. M. Growth kinetics of plant cells in suspension culture. The Netherland: Delft Univ. Technology Thesis; 1990.
Wilson, G. A simple and inexpensive design of chemostat enabling steadystate growth ofAcer pseudoplatamus L. cells under phosphate-limiting conditions. Ann. Bot. 40:919–932; 1976.
Author information
Authors and Affiliations
Additional information
Presented in the Session-in-Depth Batch Production and Fermentation at the 1991 World Congress on Cell and Tissue Culture, Anaheim, California, June 16–20, 1991.
Rights and permissions
About this article
Cite this article
ten Hoopen, H.J.G., van Gulik, W.M. & Heijnen, J.J. Continuous culture of suspended plant cells. In Vitro Cell Dev Biol – Plant 28, 115–120 (1992). https://doi.org/10.1007/BF02823058
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02823058