Summary
Hancornia speciosa fruit is highly desired for the juice and ice cream industry in tropical regions. A rapid reduction in germination ability ofH. speciosa seeds has been a problem for its large-scale cultivation. This paper describes anin vitro technique that may lead to an alternative propagation method forH. speciosa. Shoot apices and nodal segments from aseptically germinated young embryos were cultivatedin vitro on. Murashige and Skoog (1962) medium supplemented with growth regulators. Shoot multiplication was maintained by sequential subculture of shoot tips and nodal segments. N6-benzyladenine was the most effective cytokinin for the induction of shoot growth. N6-furfurylamino-purine, at various concentrations, yielded multiplication rates sevenfold lower than the highest multiplication rate found with N6-benzyladenine. Increased root initiation rate and root elongation was observed with the presence of γ-(indole-3) butyric acid in the half-strength Murashige and Skoog culture medium, especially at 10μM. N6-benzyladenine strongly inhibited rooting, even in the presence of γ-(indole-3) butyric acid. Thein-vitro-raised rooted plantlets were acclimatized to greenhouse environment through progressive reduction in relative humidity and later transplanted to the field.
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Pereira-Netto, A.B. In vitro propagation ofHancornia speciosa, a tropical fruit tree. In Vitro Cell.Dev.Biol.-Plant 32, 253–256 (1996). https://doi.org/10.1007/BF02822696
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DOI: https://doi.org/10.1007/BF02822696