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Structural studies and physiological activity of lemnan, a pectin fromLemna minor L

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Abstract

A pectic polysaccharide, lemnan, was isolated from freshly collected duckweedLemna minor L. Its sugar chain was shown to be mainly composed of the residues ofD-galacturonic acid (64%), galactose, arabinose, xylose, andD-apiose, a branched chain sugar. The high content ofD-apiose (25%) indicated that lemnan is an apiogalacturonan type pectin similar to zosteran, a pectic polysaccharide from a sea phanerogam of the Zosteraceae family. The results of partial acidic hydrolysis, pectinase digestion, and NMR studies of lemnan demonstrated that its macromolecule contains regions of the linear α-1,4-D-galacturonan and branched apiogalacturonan. The side chains of apiogalacturonan were found to be formed of single and 1,5-linked residues ofD-apiofuranose attached to 2- and 3-positions of theD-galacturonic acid residues of the apiogalacturonan backbone. Lemnan was shown to exhibit an immunomodulatory effect activating the system of phagocytosis.

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Abbreviations

TFA:

trifluoroacetic acid

References

  1. Ovodova, R.G., Vas’kovsky, V.E., and Ovodov, Yu.S.,Carbohydr. Res., 1968, vol. 6, pp. 328–332.

    Article  CAS  Google Scholar 

  2. Ovodova, R.G. and Ovodov, Yu.S.,Carbohydr. Res., 1969, vol. 10, pp. 387–390.

    Article  CAS  Google Scholar 

  3. Ovodov, Yu.S., Ovodova, R.G., Bondarenko, O.D., and Krasikova, I.N.,Carbohydr. Res., 1971, vol. 18, pp. 311–318.

    Article  CAS  Google Scholar 

  4. Ovodov, Yu.S., Ovodova, R.G., Shibaeva, V.I., and Mikheyskaya, L.V.,Carbohydr. Res., 1975, vol. 42, pp. 197–199.

    Article  CAS  Google Scholar 

  5. Ovodov, Yu.S., Ovodova, R.G., and Sorochan, V.D.,Food Hydrocoll., 1992, vol. 6, pp. 125–128.

    Google Scholar 

  6. Loenko, Yu. N., Artyukov, A.A, Kozlovskaya, E.P., Miroshnichenko, V.A., and Elyakov, G.B.,Zosterin, Vladivostok: Dal’nauka, 1997, pp. 41–145.

    Google Scholar 

  7. Ovodov, Yu.S.,Bioorg. Khim., 1998, vol. 24, pp. 483–501.

    PubMed  CAS  Google Scholar 

  8. Hart, D.A. and Kindel, P.K.,Biochem. J., 1970, vol. 116, pp. 569–579.

    PubMed  CAS  Google Scholar 

  9. Kindel, P.K., Cheng, L., and Ade, R.B.,Phytochemistry, 1996, vol. 41, pp. 719–723.

    Article  CAS  Google Scholar 

  10. Cheng, L. and Kindel, P.K.,Carbohydr. Res., 1997, vol. 301, pp. 205–212.

    Article  CAS  Google Scholar 

  11. Ovodov, Yu.S., Ovodova, R.G., Popov, S.V., and Bil’kova, T.B., Abstracts of Papers,XIX Intern. Carbohydr. Symp., San Diego, 1998, p. DP041.

  12. Catoire, L., Goldberg, R., Pierron, M., Morvan, C., and Hervé du Penhoat, C.,Eur. Biophys. J., 1998, vol. 27, pp. 127–136.

    Article  PubMed  CAS  Google Scholar 

  13. Odonmażig, P., Badga, D., Ebringerova, A., and Alföldi, J.,Carbohydr. Res., 1992, vol. 226, pp. 353–360.

    Article  Google Scholar 

  14. Bock, K. and Pedersen, C.,Adv. Carbohydr. Chem. Biochem., 1983, vol. 83, pp. 27–66.

    Google Scholar 

  15. Patt, S.L. and Shoolery, J.N.,J. Magn. Reson., 1982, vol. 46, pp. 535–539.

    CAS  Google Scholar 

  16. Blindar’, V., Zubrikhina, G., Kruglova, N., and Nikitina, T.,Clin. lab. diagn., 1996, no. 2, pp. 18–20.

  17. Pinegin, B.V., Butakov, A.A., and Shchel’tsina, T.L.,Ecological Immunology, Khaitov, R.M., Pinegin, B.V., and Istamov, K.I., Eds., Moscow: VNIRO, 1995, pp. 106–162.

    Google Scholar 

  18. Khant, S.,Lymphocytes: A Practical Approach, Klaus, G.G.B., Ed., Oxford, Washington DC: IRL, 1987, chapter 1. Translated under the titleLimfocity. Metody, Moscow: Mir, 1990, pp. 15–65.

    Google Scholar 

  19. Prosorovskii, V., Prosorovskaya, M., and Demchenko, V.,Farmakol. Toksikol., 1978, no. 4, pp. 497–502.

  20. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J.,J. Biol. Chem., 1951, vol. 193, pp. 265–275.

    PubMed  CAS  Google Scholar 

  21. Usov, A.T., Bilan, M.I., and Klochkova, N.G.,Bot. Marina, 1995, vol. 38, pp. 43–51.

    Article  CAS  Google Scholar 

  22. York, W.S., Darvill, A.G., McNeil, M.A., Stevenson, T.T., and Albersheim, P.,Meth. Enzymol., 1985, vol. 118, pp. 3–40.

    Article  Google Scholar 

  23. Nefedov, P.P. and Lavrenko, P.N.,Transportnye metody v analiticheskoi khimii polimerov (Transport Methods in Analytical Chemistry of Polymers), Leningrad: Khimiya, 1979.

    Google Scholar 

  24. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., and Smith, F.,Anal. Chem., 1956, vol. 28, pp. 350–356.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Physiology, Komi Science Center, Ural Branch, Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982, Syktyvkar, Russia

    R. G. Ovodova, V. V. Golovchenko, S. V. Popov & Yu. S. Ovodov

  2. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii pr. 47, 117913, Moscow, Russia

    A. S. Shashkov

Authors
  1. R. G. Ovodova
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  2. V. V. Golovchenko
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  3. A. S. Shashkov
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  4. S. V. Popov
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  5. Yu. S. Ovodov
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Correspondence to R. G. Ovodova.

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Ovodova, R.G., Golovchenko, V.V., Shashkov, A.S. et al. Structural studies and physiological activity of lemnan, a pectin fromLemna minor L. Russ J Bioorg Chem 26, 669–676 (2000). https://doi.org/10.1007/BF02821835

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  • DOI: https://doi.org/10.1007/BF02821835

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