Abstract
This article is devoted to the recent advances made in the Southern blotting technique, which is used for the detection of gel-fractionated DNA molecules following transfer to a membrane. The latest practical improvements made to the techniques of Southern blotting, probe labeling, and hybridization are discussed. Among the most significant advances are: new membranes, transfer methods, probe labeling, and rapid hybridization. Detailed protocols show the application of these improvements in this widely used technique.
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Evans, M.R., Bertera, A.L. & Harris, D.W. The Southern blot. Mol Biotechnol 1, 1–12 (1994). https://doi.org/10.1007/BF02821507
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DOI: https://doi.org/10.1007/BF02821507