Skip to main content
SpringerLink
Log in

Preparation of protoplasts and regeneration of intact cells ofStreptomyces cinnamonensis

  • Brief Report
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Protoplasts were prepared and intact cells were regenerated inStreptomyces cinnamonensis— a monensin producer— to make genetic manipulations with this strain possible. 70–80% of protoplasts were formed and up to 90% of them could regenerate into intact cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baltz R.H., Matsushima P.: Protoplast fusion inStreptomyces—conditions for efficient genetic recombination and cell regeneration.J. Gen. Microbiol. 127, 137–146 (1981).

    PubMed  CAS  Google Scholar 

  • Hopwood G.D.A., Chater K.F.: Cloning inStreptomyces—systems and strategies, pp. 119–145 in J.K. Setlow, A. Hollaender (Eds.):Genetic Engineering (Principles and Methods), Vol. 4. Plenum Press, New York 1982.

    Google Scholar 

  • Hopwood D.A., Bibb M.J., Chater K.F., Kieser T., Bruton C.J., Kieser H.M., Lydiate D.J., Smith C.P., Ward J.M., Schrempf H.:Genetic Manipulation of Streptomyces (A Laboratory Manual). The John Innes Foundation, Norwich 1985.

    Google Scholar 

  • Hranueli D., Smokvina T., Alačević M.: A comparative study of protoplasts preparation and regeneration inStreptomyces rimosus andStr. lividans.Microbiologica 9, 387–392 (1986).

    PubMed  CAS  Google Scholar 

  • Ogata S., Koyama-Miyoshi Y., Hayashida S.: Transfection and transformation systems for pock-forming and thiostrepton-producingStreptomyces azureus.J. Fac. Agr. Kyushu Univ. 29, 179–188 (1985).

    CAS  Google Scholar 

  • Ogawa H., Imai S., Satoh A., Kojima M.: An improved method for the preparation of streptomycetes and micromonospora protoplasts.J. Antibiot. 36 184–186 (1983).

    PubMed  CAS  Google Scholar 

  • Okanishi M., Suzuki K., Umezawa H.: Formation and reversion of streptomycete protoplasts: Cultural conditions and morphological study.J. Gen. Microbiol. 80, 389–400 (1974).

    PubMed  CAS  Google Scholar 

  • Shirahama T., Furumai T., Okanishi M.: A modified regeneration method for streptomyces protoplasts.Agric. Biol. Chem. 45, 1724–1728 (1981).

    Google Scholar 

  • Tichý P., Klánová K., Cimburková, E., Vaněk Z., Alyiokhova T.: Plasmids inStreptomyces cinnamonensis, a monensin producer.Biotechnol. Lett. 4, 437–440 (1982).

    Google Scholar 

  • Yamomoto H., Maurer K.H., Hutchinson C.R.: Transformation ofStreptomyces erythreus.J. Antibiot. 39, 1304–1313 (1986).

    Google Scholar 

  • Yamashita F., Hotta K., Kurasawa S., Okami Y., Umezawa H.: New antibiotic-production generated by protoplast fusion treatment betweenS. griseus andS. tenjimariensis.J. Antibiot. 38, 58–63 (1985).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of General Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague 4

    Z. Jandová & P. Tichý

Authors
  1. Z. Jandová
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Tichý
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jandová, Z., Tichý, P. Preparation of protoplasts and regeneration of intact cells ofStreptomyces cinnamonensis . Folia Microbiol 35, 456–459 (1990). https://doi.org/10.1007/BF02821416

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02821416

Keywords

Search

Navigation