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Production and characteristics of the exocellular polysaccharide of a mutantMycobacterium strain

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Abstract

Mutant strains ofMycobacterium sp. V-649 producing highly mucous colonies on a solid cultivation medium were prepared after treatment with N-methyl-N′-nitro-N-nitrosoguanidine and production of the exocellular polysaccharide was tested. The strains were cultivated in media with suitable sugar sources under submerged conditions. It was found thatMycobacterium sp. V649/15 produces a maximum of 15–19% polymer after a 5–6-d cultivation. Gas chromatography indicated that the exocellular polysaccharide produced by this strain is of glucan type.

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Koníček, J., Daraselia, G.Y., Koníčková-Radochova, M. et al. Production and characteristics of the exocellular polysaccharide of a mutantMycobacterium strain. Folia Microbiol 35, 394–401 (1990). https://doi.org/10.1007/BF02821408

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