Abstract
α-Amylase was found to be the main protein secreted byBacillus subtilis, corresponding to 90, 87 and 60% of total extracellular proteins at 30, 40 and 45°C, respectively. A change in temperature can affect the pattern of proteins secreted as detected by gel electrophoresis.14C-Leucine incorporation into extracellular proteins and their proportion at the end of the growth phase was higher at 30°C than that at 40 or 45°C. The effect of temperature on α-amylase synthesis as determined by its enzymic activity and on the extracellular protein synthesis followed a similar pattern.
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Antranikian G., Herzberg C., Mayer F., Gottschalk G.: Changes in the cell envelope structure ofClostridium sp. strain EM1 during massive production of α-amylase and pullulanase.FEMS Microbiol. Lett. 41, 193–197 (1987).
Bradford M.M.: A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal. Biochem. 72, 248–254 (1976).
Chaloupka J., Strnadová M.: Kinetics of protein turnover in growing cells ofBacillus megaterium.J. Gen. Microbiol..128, 1003–1008 (1982).
De Mendoza D., Cronan J.E. Jr.: Thermal regulation of membrane lipid fluidity in bacteria.Trends Biochem. Sci. 8, 49–52 (1983).
Estrela A.I., Lemos M., Spencer-Martins I.: A note on the effect of growth temperature on the production of amylases by the yeastLipomyces kononenkoae.J. Appl. Bacteriol. 52, 465–467 (1982).
Hao J.: The regulation by growth temperature of the α-amylase gene epxression inBacillus subtilis A18 cells.PhD Thesis. Institute of Microbiology, Czech. Acad. Sci, Prague 1988.
Hao J., Pazlarová J., Strnadová M., Tichý P., Chaloupka J.: Effect of temperature on α-amylase formation and DNA replication inBacillus subtilis.Folia Microbiol. 33, 230–233 (1988).
Laemmli U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature.227, 680–685 (1970).
Madi E., Antranikian G., Ohmiya K., Gottschalk G.: Thermostable amylolytic enzymes from a newClostridium isolate.Appl. Environ. Microbiol..53, 1661–1667 (1987).
Nagata Y., Yamaguchi K., Maruo B.: Genetic and biochemical studies on cell-bound α-amylase inBacillus subtilis Marburg.J. Bacteriol..119, 425–430 (1974).
Pazlarová J.: Exocellular protein and α-amylase secretion, pp. 139–142 inExtracellular Enzymes of Microorganisms (J. Chaloupka, V. Krumphanzl, Eds.). Plenum Press, New York-London 1987.
Potier P., Hipkiss A.R., Kushner D.J.: Protein turnover in a psychotrophic bacterium.Arch. Microbiol. 142, 28–33 (1985).
Potier P., Drevet P., Gount A., Hipkiss A.R.: Protein turnover in a psychotrophic bacterium: Proteolytic activity in extracts of cells grown at different temperatures.FEMS Microbiol. Lett..44, 267–271 (1987).
Pretorius I.S., De Kock M.J., Britz T.J., Potgieter H.J., Lategan P.M.: Numerical taxonomy of α-amylase producingBacillus species.J. Appl. Bacteriol..60, 351–360 (1986).
Priest F.G., Thirunavukkarasu M.: Synthesis and location of α-amylase and α-glucosidase inBacillus licheniformis grown in batch and continuoous culture.J. Appl. Bacteriol..58, 381–390 (1985).
Srivastava R.A.K., Baruah J.N.: Culture conditions for production of thermostable amylase byBacillus stearothermophilus.Appl. Environ. Microbiol.,52, 179–184 (1986).
Strnadová M., Prasad R., Kučerová H., Chaloupka J.: Effect of temperature on growth and protein turnover inBacillus megaterium.J. Basic Microbiol..5, 289–298 (1986).
Svobodová J., Svoboda P.: Membrane fluidity inBacillus subtilis. Physical change and biological adaptation.Folia Microbiol..33, 161–169 (1988).
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Hao, J., Pazlarová, J., Strnadová, M. et al. Regulation of extracellular proteins and α-amylase secretion by temperature inBacillus subtilis . Folia Microbiol 34, 179–184 (1989). https://doi.org/10.1007/BF02821290
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DOI: https://doi.org/10.1007/BF02821290