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Alterations in the surface charge of heart muscle cells during interaction withTrypanosoma cruzi

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Abstract

The surface charge of heart muscle cells (HMC) andTrypanosoma cruzi trypomastigotes was estimated during their interaction by means of zeta potential (ZP). Metacyclic and bloodstream trypomastigote, but not amastigote forms, are able to decrease the surface charge of HMC as well as other nonphagocytic cells. However, no alteration could be detected onT. cruzi-infected macrophage cell line. Trypomastigote forms collected from the supernatant after 20 h of contact with HMC also have their ZP value decreased. The analysis of the surface components of both the parasite and HMC involved in such interaction was also carried out. Assays concerning the kinetics of the cell-parasite interaction demonstrated the influence of parasite surface anionogenicity during its interaction with HMC. The binding of bloodstream forms to HMC was enhanced after their incubation with cationized ferritin (CF), whereas phospholipase C and neuraminidase treatments improved and trypsin treatment inhibited parasite uptake in HMC. Conversely, the incubation of HMC with phospholipase C impaired, and with trypsin enhanced, the interiorization of the parasites. These results suggest that trypomastigote forms ofT. cruzi may process the surface of HMC and its own surface either by removing molecules or by exposing ligands for their internalization.

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  1. Departamento de Ultraestrutura e Biologia Celular/Instituto Oswaldo Cruz, FIOCRUZ, Av. Brazil 4365, 21045-900, Rio de Janeiro, Brazil

    Maria de Nazaré C. Soeiro & Maria de Nazareth Leal de Meirelles

  2. Programa de Biologia Celular e Parasitologia, Instituto de Biofisica Carlos Chagas Filho, UFRJ, CCS-Bloco G, Rio de Janeiro, Brazil

    Fernando Costa e Silva Filho

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Soeiro, M.d.N.C., Filho, F.C.e.S. & de Meirelles, M.d.N.L. Alterations in the surface charge of heart muscle cells during interaction withTrypanosoma cruzi . Cell Biophysics 26, 21–44 (1995). https://doi.org/10.1007/BF02820885

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