Biological Trace Element Research

, Volume 62, Issue 1–2, pp 7–16 | Cite as

Changes in blood chemistry, hematology, and histology caused by a selenium/vitamin E deficiency and recovery in chicks

  • Amy Bartholomew
  • David Latshaw
  • David E. Swayne


Exudative diathesis, a condition caused by a selenium (Se)/vitamin E deficiency, was studied in chicks. Trios of chicks that showed clinical signs of exudative diathesis were matched for severity. One was injected subcutaneously with 0.5 mL distilled water, and the other two received 15 μg of Se in 0.5 mL distilled water. A chick fed a diet with supplemental Se also received 0.5 mL distilled water. Blood was collected from three chicks 2 d after injection, and from the other chick, 6 d after injection. After blood was collected, pectoral muscle and bone marrow were collected. Deficient chicks showed varying degrees of necrosis in pectoral muscle, whereas recovring chicks had extensive fibrosis in pectoral muscle. An analysis of blood showed differences in CO2, glucose, Se, glutathione peroxidase, alanine aminotransferase, aspartate aminotransferase, and creatine kinase. Heterophils and monocytes were increased in deficient chicks; lymphocytes, basophils, and hemoglobin decreased. After 6 d of recovery, all of the changes noted above were correcting toward normal. Eosinophils, in contrast, were unaffected by a deficiency, but increased in recovering chicks. It is hypothesized that cytokines associated with the inflammatory response accentuate the clinical signs of exudative diathesis.

Index Entries

Chicks selenium inflammatory response necrosis exudative diathesis 


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  1. 1.
    H. Dam and J. Glavind, Alimentary exudative diathesis,Nature 142, 1077–1078 (1938).CrossRefGoogle Scholar
  2. 2.
    H. Dam and J. Glavind, Alimentary exudative diathesis, a consequence of E-avitaminosis.Nature 143, 810–811 (1939).CrossRefGoogle Scholar
  3. 3.
    E. L. R. Stokstad, E. L. Patterson, and R. Milstrey, Factors which prevent exudative diathesis in chicks on Torula yeast diets [Abstract],Poultry Sci. 36, 1160 (1957).Google Scholar
  4. 4.
    M. L. Scott, J. G. Bieri, G. M. Briggs, and K. Schwarz, Prevention of exudative diathesis by Factor 3 in chicks on vitamin E-deficient Torula yeast diets [Abstract],Poultry Sci. 36, 1155 (1957).Google Scholar
  5. 5.
    T. Noguchi, A. H. Cantor, and M. L. Scott, Mode of action of selenium and vitamin E in prevention of exudative diathesis in chicks,J. Nutr. 103, 1502–1511 (1973).PubMedGoogle Scholar
  6. 6.
    M. L. Scott, M. C. Nesheim, and R. J. Young,Nutrition of the Chicken, 3rd ed., M. L. Scott and Associates, Ithaca, pp. 350–353 (1982).Google Scholar
  7. 7.
    T. S. Koh and T. H. Benson, Critical re-appraisal of fluorometric method for determination of selenium in biological materials,J. Assoc. Off. Anal. Chem. 66, 918–926 (1983).PubMedGoogle Scholar
  8. 8.
    R. P. Burk and R. A. Lawrence, Glutathione-peroxidase activity in selenium deficient rat liver,Biochem. Biophys. Res. Commun. 71, 952–958 (1976).PubMedCrossRefGoogle Scholar
  9. 9.
    C. Riddell, Muscular system, inAvian Histopathology, American Association of Avian Pathologists, Kennett Square, PA, pp. 27–30 (1987).Google Scholar
  10. 10.
    C. Riddell, Cardiovascular system, inAvian Histopathology, American Association of Avian Pathologists, Kennett Square, PA, pp. 31–36 (1987).Google Scholar
  11. 11.
    R. E. Austic and M. L. Scott, Nutritional diseases, inDiseases of Poultry, 9th ed., B. W. Calnek, H. J. Barnes, C. W. Beard, W. M. Reid, and H. W. Yoder, Jr. eds., Iowa State University Press, Ames, IA, pp. 45–71 (1991).Google Scholar
  12. 12.
    J. Kuby,Immunology, 2nd ed., W.H. Freeman, New York, pp. 7–15,313–316 (1994).Google Scholar
  13. 13.
    L. R. McDowell, Selenium, inMinerals in Animal and Human Nutrition, Academic, New York, pp. 294–332 (1992).Google Scholar

Copyright information

© Humana Press Inc 1998

Authors and Affiliations

  • Amy Bartholomew
    • 1
  • David Latshaw
    • 1
  • David E. Swayne
    • 2
  1. 1.Department of Animal SciencesOhio State UniversityColumbus
  2. 2.USDA/ARS/SEPRLAthens

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