Abstract
Objective
To investigate the relationship between P120ctn translocation and hepatocellular carcinoma cell malignant features and the relationship between p 120ctn and β-catenin translocation in cell signaling.
Methods
Human hepatocellular carcinoma cells were over expressed with p 120ctn isoform 3A using a DNA transfection method. The effects of transfection and expression of p 120{ctn} and its binding capacity to E-cadherin were examined using immunoprecipitation and immunoblotting methods. p 120ctn subcellular localization and its relation with β-catenin were detected using immunofluorescent microscopy. p 120{ctn} phosphorylation was produced by EGF treatment. Cell adhesion, cell migration and cell proliferation were also examined in this study.
Results
We found that p120{ctn} expression was increased after transfection and the binding capacity of P 120{ctn} to E-cadherin was enhanced. Tyrosine phosphorylation of p 120{ctn} increased after transfection and EGF treatment. p 120{ctn} and β-catenin cellular localization displayd a membrane and cytoplasmic expression pattern, but they translocated into the nucleus for relocalization after p 120{ctn} overexpression plus EGF stimulation. Cell adhesion ability was increased and migration ability reduced after transfection without EGF. Following transfection without EGF cellular proliferation was reduced, but increased after EGF treatment.
Conclusion
Our results suggest that p 120ctn plays an important role in hepatocellular carcinoma cell adhesion, migration and proliferation. In addition there is a relationship between p 120ctn and β-catenin subcellular localization and signaling.
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This work was supported by the National Natural Science Foundation of China (No. 30160096).
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Huang, H., Nong, C., He, W. et al. p120 catenin translocation is involved in enhancement of hepatoma cellular malignant features. Chin. J. Clin. Oncol. 2, 693–699 (2005). https://doi.org/10.1007/BF02819534
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DOI: https://doi.org/10.1007/BF02819534