Abstract
Using both monoclonal and polyclonal antibodies against mammalian plectin (multifunctional protein cross-linking cytoskeletal structures, mainly intermediate filaments, in mammalian cells), several putative isoforms of plectin-like proteins were found in protein extracts from the green algaChlamydomonas eugametos (Volvocales). Immunofluorescence and immunoblotting revealed that some of the plectin-like proteins were present in perinuclear region or localized near the cell wall, probably being attached to the cytoplasmic membrane.
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Andrä K., Nikolic B., Stocher M., Drenckhahn D., Wiche G.: Not just scaffolding: plectin regulates actin dynamics in cultured cells.Genes Dev. 12, 3442–3451 (1998).
Berger S., Wittke W., Traub P.: Occurrence of proteinaceous 10-nm filaments throughout the cytoplasm of algae of the orderDasycladales.Exp. Cell Res. 240, 176–186 (1998).
Beven A., Guan Y.H., Peart J., Cooper C., Shaw P.: Monoclonal antibodies to plant nuclear matrix reveal intermediate filament-related components within the nucleus.J. Cell Sci. 98, 293–302 (1991).
Dawson P.J., Hulme J.S., Lloyd C.W.: Monoclonal antibody to intermediate filament antigen cross-reacts with higher plant cells.J. Cell Biol. 100, 1793–1798 (1985).
Eger A., Stockinger A., Wiche G., Foisner R.: Polarization-dependent association of plectin with desmoplakin and the lateral submembrane skeleton in MDCK cells.J. Cell Sci. 110, 1307–1316 (1997).
Foisner R.: Dynamic organization of intermediate filaments and associated proteins during the cell cycle.BioEssays 19, 297–305 (1997).
Foisner R., Bohn W., Mannweiler K., Wiche G.: Distribution and ultrastructure of plectin arrays in subclones of rat glioma C6 cells differing in intermediate filament protein (vimentin) expression.J. Struct. Biol. 115, 304–317 (1995).
Foisner R., Feldman B., Sander L., Seifert G., Artlieb U., Wiche G.: A panel of monoclonal antibodies to rat plectin: distinction by epitope mapping and immunoreactivity with different tissues and cell lines.Acta Histochem. 96, 421–438 (1994).
Foisner R., Feldman B., Sander L., Wiche G.: Monoclonal antibody mapping of structural and functional plectin epitopes.J. Cell Biol. 112, 397–405 (1991).
Foisner R., Malecz N., Dressel N., Stadler C., Wiche G.: M-phase-specific phosphorylation and structural rearrangement of the cytoplasmic cross-linking protein plectin involve p34cdc2 kinase.Mol. Biol. Cell 7, 273–288 (1996).
Foisner R., Traub P., Wiche G.: Protein kinase A- and protein kinase C-regulated interaction of plectin with lamin B and vimentin.Proc. Nat. Acad. Sci. USA 88, 3812–3816 (1991).
Frederick S.E., Mangan M.E., Carey J.B., Gruber P.J.: Intermediate filament antigens of 60 and 65 kDa in the nuclear matrix of plants: their detection and localization.Exp. Cell Res. 199, 213–222 (1992).
Fuchs P., Zorer M., Reznickzek G.A., Spazierer D., Oehler S., Castanon M.J., Hauptmann R., Wiche G.: Unusual 5′ transcript complexity of plectin isoforms: novel tissue-specific exons modulate actin binding activity.Hum. Mol. Genet. 8, 2461–2472 (1999).
Goodbody K.C., Hargreaves A.J., Lloyd C.W.: On the distribution of microtubule-associated intermediate filament antigens in plant suspension cells.J. Cell Sci. 93, 427–438 (1989).
Hargreaves A.J., Dawson P.J., Butcher G.W., Larkins A., Goodbody K.C., Lloyd C.W.: A monoclonal antibody raised against cytoplasmic fibrillar bundles from carrot cells, and its cross-reaction with animal intermediate filaments.J. Cell Sci. 92, 371–378 (1989).
Hašek J., Streiblová E.: Fluorescence microscopy methods, pp. 391–405 in I. Evans (Ed.):Methods in Molecular Biology: Yeast Protocols. Humana Press, Totowa (USA) 1996.
Herrmann H., Wiche G.: Plectin and IFAP-300K are homologous proteins binding to microtubule-associated proteins 1 and 2 and to the 240-kilodalton subunit of spectrin.J. Biol. Chem. 262, 1320–1325 (1987).
Laemmli U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227, 680–685 (1970).
Lorenz M., Bisikirska B., Hanus-Lorenz B., Strzalka K., Sikorski A.F.: Proteins reacting with anti-spectrin antibodies are present inChlamydomonas cells.Cell Biol. Internat. 19, 83–90 (1995).
Malecz N., Foisner R., Stadler C., Wiche G.: Identification of plectin as a substrate of p34cdc2 kinase and mapping of a single phosphorylation site.J. Biol. Chem. 271, 8203–8208 (1996).
Parke J.M., Miller C.C., Cowell I., Dodson A., Dowding A., Downes M., Duckett J.G., Anderton B.J.: Monoclonal antibodies against plant proteins recognize animal intermediate filaments.Cell Motil. Cytoskelet. 8, 312–323 (1987).
Skalli O.C., Chou Y.-H., Goldman R.D.: Cell cycle-dependent changes in the organization of an intermediate filament-associated protein: correlation with phosphorylation by p34cdc2.Proc. Nat. Acad. Sci. USA 89, 11959–11963 (1992).
Stanbridge E.J., Der C.J., Doersen C.J., Nishimi R.Y., Peehl D.M., Weissman B.E., Wilkinson J.E.: Human cell hybrids: analysis of transformation and tumorigenicity.Science 215, 252–259 (1982).
Steinböck F.A., Wiche G.: Plectin: a cytolinker by design.Biol. Chem. 380, 151–158 (1999).
Svitkina T.M., Verkhovsky A.B., Borisy G.B.: Plectin sidearms mediate interactions of intermediate filaments with microtubules and other components of the cytoskeleton.Biol. Bull. 194, 409–410 (1998).
Towbin H., Staehelin T., Gordon J.: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proc. Nat. Acad. Sci. USA 76, 4350–4354 (1979).
Wiche G.: Plectin: general overview and appraisal of its potential role as a subunit protein of the cytomatrix.Crit. Rev. Biochem. Mol. Biol. 24, 41–66 (1989).
Wiche G.: Role of plectin in cytoskeleton organization and dynamics.J. Cell Sci. 111, 2477–2486 (1998).
Wiche G., Baker M.A.: Cytoplasmic network arrays demonstrated by immunolocalization using antibodies to a high molecular weight protein present in cytoskeletal preparations from cultured cells.Exp. Cell Res. 138, 15–19 (1982).
Yang C., Xing L., Zhai Z.: Intermediate filaments in higher plant cells and their assembly in a cell-free system.Protoplasma 171, 44–54 (1992).
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Hendrychová, J., Vítová, M., Bišová, K. et al. Plectin-like proteins are present in cells ofChlamydomonas eugametos (Volvocales) . Folia Microbiol 47, 535–539 (2002). https://doi.org/10.1007/BF02818794
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DOI: https://doi.org/10.1007/BF02818794