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Characterization of the third generation enzyme immunoassay IEA-HIV1/2-III for the detection of anti-HIV specific antibodies in human sera

  • Immunology
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Abstract

The sensitivity and specificity of the developed anti-HIV1/2 third generation enzyme immunoassay, the IEA-HIV1/2-III, was examined. The test system for the detection of anti-HIV antibodies included peroxidase-conjugated HIV-specific recombinant Gag protein fragments (epitopes of p24 and p17 proteins), Env-1 (epitopes of p41 and p120 proteins), and Env-2 (p36 epitopes). Sensitivity was evaluated with 346 sera from HIV1-seropositive subjects, Anti-HIV1 Low Titer panels no. 10 and PRB-106 and seropositive panel PRB-931 in comparison with other third- and second-generation assays. The IEA-HIV1/2-III assays are characterized with high sensitivity comparable to the other third generation assays and the better sensitivity with respect to the second generation test-kit to determine HIV-specific antibodies in human sera. The specificity was determined using three hundred sixty-seven potentially cross-reactive samples (but negative for anti-HIV1/2). Only one specimen among them was reactive by IEA-HIV1/2-III.

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Authors and Affiliations

  1. Scientific-Production Enterprise Diaproph-Med, Kiev, Ukraine

    G. Rayevskaya, V. G. Pilipenko & R. M. Chumak

  2. University of Veterinary Medicine, 040 01, Košice, Slovakia

    L. Tkáčiková & I. Mikula

  3. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 252 143, Kiev, Ukraine

    N. Y. Spivak

  4. Neuroimmunology Institute, Slovak Academy of Sciences, 842 46, Bratislava, Slovakia

    I. Mikula

Authors
  1. G. Rayevskaya
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  2. V. G. Pilipenko
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  3. L. Tkáčiková
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  4. N. Y. Spivak
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  5. I. Mikula
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  6. R. M. Chumak
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Rayevskaya, G., Pilipenko, V.G., Tkáčiková, L. et al. Characterization of the third generation enzyme immunoassay IEA-HIV1/2-III for the detection of anti-HIV specific antibodies in human sera. Folia Microbiol 45, 577–580 (2000). https://doi.org/10.1007/BF02818730

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  • DOI: https://doi.org/10.1007/BF02818730

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