Abstract
The effect ofO-ethyl-S-(2-diisopropylaminoethyl) methylthiophosphonate on rumen bacteria and rumen protozoa was investigated in sheep (after premedication with clinoptilolite-rich zeolite and without that premedication). In control animals a decrease in the total concentration of rumen protozoa was observed 3–7 d after intoxication (particularly in small and large ones). In clinoptilolite-rich-zeolite-treated animals only a slight decrease in protozoan numbers occurred during the first hours after the intoxication. Similarly, in every category of rumen bacteria marked differences between the groups were recorded, particularly in concentration of lipolytic bacteria. The results suggest some protective effect of clinoptilolite-rich zeolite for rumen microbiota against the organophosphate poison.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abou-Akkada A.R., Blackburn T.H.: Some observations on the nitrogen metabolism of rumen proteolytic bacteria.J. Gen. Microbiol.31, 461–472 (1963).
Blackburn T.H., Hobson P.N.: Further studies on the isolation of proteolytic bacteria from the sheep rumen.J. Gen. Microbiol.29, 69–74 (1962).
De Bleecker J., Van Den Neucker K., Willems J.: The intermediate syndrome in organophosphate poisoning: presentation of a case and review of the literature.J. Toxicol. Clin. Toxicol.30, 321–329 (1992).
Chambers J.E., Levi P.E.:Organophosphates: Chemistry, Fate, and Effects. Academic Press, San Diego (CA) 1992.
Hasan H.A.H.: Fungal utilization of organophosphate pesticides and their degradation byAspergillus flavus andA. sydowii in soil.Folia Microbiol.1, 77–84 (1999).
Hobson P.N.: Rumen bacteria.Meth. Microbiol.3B, 33–174 (1969).
Hobson P.N., Mann S.O.: The isolation and culture of glycerol-fermenting and lipolytic bacteria from the rumen of the sheep.J. Gen. Microbiol.25, 227–238 (1961).
Hoskin F.C., Walker J.E.: Enzymatic hydrolysis of tetriso as a model for the detoxication of the neurotoxic agent VX.Biol. Bull.187, 249–265 (1994).
Hungate R.E.: Mieroorganisms in the rumen of cattle fed a constant ration.Can. J. Microbiol.3, 289–293 (1957).
Kováč G.: Intoxication with organophosphates in farm animals. (In Slovak)Slov. Vet. Čas.2, 77–82 (1994).
Kováč G., Bartko P., Vrzgula L., Reichel P., Ništiar F., Mojžiš J., Seidel H.: Application of clinop intoxication in sheep, pp. 459–466 in D.W. Ming, F.A. Mumpton (Eds):Natural Zeolites '93. Internat. Comm. Natural Zeolites. Brockport, New York 1995.
Kováč G., Reichel P., Seidel H., Mudroň P.: Effects of sorbents during organophosphate intoxication in sheep.Czech J. Anim. Sci.43, 3–7 (1998).
Kozáč J., Očenáš D., Hoppan J.: Mineralogy, characteristic properties and utilization possibilities of zeolitic tuffite from Nižný Hrabovec (East Slovakia).
Kozáč J., Očenáš D.: Determination of clinoptilolite content in zeolitic tuffits.Mineralia Slov.14, 549–552 (1982).
Kurihara Y., Eadie J.M., Hobson P.N., Mann S.O.: Relationship between bacteria and ciliate protozoa in sheep rumen.J. Gen. Microbiol.51, 267–284 (1968).
Lotti M.: Cholinesterase inhibition: complexities in interpretation.Clin. Chem.41, 1814–1818 (1995).
Mojžiš J., Hrušovský J., Ništiar F., Mižík P.: Effect of zeolit A on the orally intoxication of rats with dichlorvos. (In Slovak)Acta Hyg. Epid. Microbiol. Suppl.5, 102–104 (1986).
Mojžiš J., Ništiar F., Kováč G., Mojžišová G.: Preventive effect of zeolite on intoxication of rats with VX substance. (In Slovak)Vet. Med.-Czech39, 443–449 (1994).
Moretto A., Johnson M.: Toxicology of organophosphates and carbamates.NATO ASI Series, Cell Biol. H. 13, pp. 33–48. Springer-Verlag, Berlin 1987.
Ništiar F.: Study of rumen flora of sheep fed by various diets.PhD Thesis. University of Veterinary Medicine, Košice 1980.
Ništiar F.: Pathogenesis, therapy and prevention of poisons with organophosphates.Habil. Thesis. Safárik University, Košice 1993.
Ništiar F., Hrušovský J., Mojžiš J.: Effect of some pesticides on protozoanTetrahymena pyriformis. (In Slovak)Vet. Med.29, 699–704 (1984a).
Ništiar F., Hrušovský J., Mojžiš J., Mižík P.: Distribution of dichlorvos in organism of rats and effect of clinoptilolite on the intoxication.Vet. Med.29, 689–698 (1984b).
Smith G.J.:Toxicology and Pesticide Use in Relation to Wildlife: Organophosphorus and Carbamate Compounds, pp. 33–68. C.K. Smoley, Boca Raton (FL) 1993.
Timbrell J.A.:Principles of Biochemical Toxicology, pp. 11–57. Taylor and Francis, Washington (DC) 1991.
Vargová M., Ondrašovičová O., Sasáková N., Ondrašovič M., Novák P., Krajňák M.: Effect of natural zeolite (clinoptilolite) on microbial decomposition processes in stored pig-slurry solids.Folia Microbiol.6, 729–734 (1999).
Votruba J. Pazlarová J. Chaloupka J.: Use of zeolite to controlBacillus megaterium extracellular proteinase production.Folia Microbiol.6, 613–616 (1998).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ništiar, F., Mojžiš, J., Kovác, G. et al. Influence of intoxication with organophosphates on rumen bacteria and rumen protozoa and protective effect of clinoptilolite-rich zeolite on bacterial and protozoan concentration in rumen. Folia Microbiol 45, 567–571 (2000). https://doi.org/10.1007/BF02818728
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02818728