Abstract
Starvation for carbon and energy sources accelerated the biotransformation of the anion-active surfactant dihexyl sulfosuccinate (DHS) byComamonas terrigena cells. Cloramphenicol (Cm) added at different time intervals to non-starved cells inhibited the DHS transformation. The largest difference between cells treated and non-treated by Cm was observed for a 16-h-starvation period. Protein synthesisde novo during starvation enhanced the DHS biotransformation efficiency. A partial transformation of DHS in the presence of Cm indicated the constitutive character of enzymes involved in primary DHS biodegradation.
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Chmelárová Ž., Závadská I., Húska J., Tóth D.: Dihexyl sulfosucinate biodegradation by mixed cultures.Folia Microbiol. 45, 491–492 (2000).
Fegatella F., Caviccholi R.: Physiological responses to starvation in the marine oligotrophic ultramicrobacteriumSphingosomonas sp. strain RB 2256.Appl. Environ. Microbiol. 66, 2037–2044 (2000).
Gregorová D., Augustín J., Vrbanová A., Sládeková D., Cserháti T.: Primary biodegradation of a series of alkyl sulfosucinates by mixed bacterial culture.Folia Microbiol. 44, 323–327 (1999).
Hartke A., Giard J.-Ch., Laplace J.-M., Auffray Y.: Survival ofEnterococcus faecalis in an oligotrophic microcosm: changes in morphology, development of general stress resistance, and analysis of protein synthesis.Appl. Environ. Microbiol. 64, 4238–4245 (1998).
Hayashi K.: A rapid determination of sodium dodecylsulphate with methylene blue.Anal. Biochem. 67, 503–506 (1975).
Herbraud M., Dubois E., Potier P., Labadie J.: Effect of growth temperatures on the protein levels in a psychrotrophic bacteriumPseudomonas fragi.J. Bacteriol. 13, 4017–4024 (1994).
Húska J., Závadská I., Tóth D.: Effect of carbon starvation of bacteria on acceleration of surfactant biotransformation.Biologia 52, 753–758 (1997).
Kjelleberg S., Albertson N., Flärdh K., Holmquist L., Jouper-Jaan A., Marouga R., Ostling J., Svenblad B., Weichart D.: How do non-differentiating bacteria adapt to starvation?Antonie van Leeuwenhoek 63, 333–341 (1993).
Kolter R., Siegele D.A., Tormo A.: The stationary phase of the bacterial life cycle.Ann. Rev. Microbiol. 47, 855–874 (1993).
Matin A.: Molecular analysis of starvation stress inEscherichia coli.FEMS Microbiol. Ecol. 74, 185–195 (1990).
Morton D.S., Oliver J.D.: Induction of carbon starvation-induced proteins inVibrio vulnificus.Appl. Environ. Microbiol. 10, 3653–3659 (1994).
Ostling J., Holmquist L., Flärdh K., Svenblad B., Jouper-Jaan A., Kjelleberg S.: Starvation and recovery ofVibrio, pp. 103–127 in S. Kjelleberg (Ed.)Starvation in Bacteria, Plenum Press, New York 1993.
Prokšová M., Augustín J., Vrbanová A.: Enrichment, isolation and characterization of dialkyl sulfosuccinate degrading bacteriaComamonas terrigena N3H andComamonas terrigena N1C.Folia Microbiol. 42, 635–639 (1997).
Roig M.G., Pedraz M.A., Sanchez J.M., Húska J., Tóth D.: Sorption isoterms and kinetics in the primary biodegradation of anionic surfactants by immobilized bacteria: II.Comamonas terrigena N3HJ. Mol. Cat. B. Enzym. 4, 271–281 (1998).
Siegele D.A., Kolter R.: Life after log.J. Bacteriol. 174, 345–348 (1992).
Smeulders M.J., Keer J., Speight R.A., Williams H.D.: Adaptation ofMyxobacterium smegmatis to stationary phase.J. Bacteriol. 181, 270–283 (1999).
Srinivasan S., Ostling J., Charlton T., Nys R.D., Takayama K., Kjelleberg S.: Extracellular signal molecule(s) involved in the carbon starvation response of marineVibrio sp. strain S14.J. Bacteriol. 180, 201–209 (1998).
Tayne W., Lavernan A., Bohland M., Braster M., Ritterhaus S., Groeneweg J., van Verseveld H.D.: Maintenance energy demand in starvation recovery dynamics ofNitrosomonas europaea andNitrobacter winogradskyi cultivated in retentostat with complete biomass retention.Appl. Environ. Microbiol. 65, 2471–2477 (1999).
Tóth D., Húska J., Závadská I., Dobrotová M.: Effect of bacterial starvation on surfactant biotransformation.Folia Microbiol. 41, 477–479 (1996).
Truex M.J., Brockmann F.J., Johnstone D.L., Frederickson J.K.: Effect of starvation on induction of quinoline degradation for a subsurface bacterium in a continuous-flow column.Appl. Environ. Microbiol. 8, 2386–2392 (1992).
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Chmelárová, Ž., Závadská, I., Húska, J. et al. Effect of starvation and chloramphenicol on acceleration of bacterial dihexyl sulfosuccinate biotransformation. Folia Microbiol 45, 493–495 (2000). https://doi.org/10.1007/BF02818716
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DOI: https://doi.org/10.1007/BF02818716