Abstract
The production of laccase in liquid cultures of the white-rot fungusPleurotus ostreatus was highly variable. During the first days of cultivation, the relative variability was as high as 80–100% and it decreased to 30% in the course of cultivation. The main source of variability was assumed to be the independent development of enzyme activity in individual cultures. Cultures with high laccase production showed also high production of the other ligninolytic enzyme—Mn-dependent peroxidase. The variability was probably due to the source of inoculum, deactivation of the enzyme in culture liquid and genetic variations among the cultures. Variability of laccase activities was lower during solid-state fermentation on wheat straw and during the growth in nonsterile soil.
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Abbreviations
- Avg:
-
average
- CCBAS:
-
Culture Collection of Basidiomycetes (Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague)
- Lac:
-
laccase
- LOM:
-
loss of organic matter
- MnP:
-
manganese peroxidase
- RSE:
-
relative standard error
- SE:
-
standard error
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Baldrian, P., Gabriel, J. Variability of laccase activity in the white-rot basidiomycetePleurotus ostreatus . Folia Microbiol 47, 385–390 (2002). https://doi.org/10.1007/BF02818695
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DOI: https://doi.org/10.1007/BF02818695