Skip to main content
Log in

Content of indoleacetic acid and its metabolism in root nodules ofMelilotus alba

  • Papers
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

The root nodules ofMelilotus alba, a leguminous fodder herb, contain a high amount of indoleacetic acid (IAA). The tryptophan pool present in the nodule might serve as a source for the IAA production. Metabolism of IAA in the nodules was evidenced by the presence of IAA-metabolizing enzymes, IAA oxidase and peroxidase. A high amount of IAA was produced by the symbiont isolated from the nodules in culture, when supplemented with tryptophan. For IAA production, the bacteria preferred thel-isomer over thedl- ord-isomer of tryptophan. The possible role of nodular IAA production on the legume-Rhizobium symbiosis is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ambasta S.P.:The Useful Plants of India, p. 362. Council of Scientific and Industrial Research, New Delhi (India) 1986.

    Google Scholar 

  • Badenoch-Jones J., Rolfe B.G., Letham D.S.: Phytohormones,Rhizobium mutants, and nodulation in lugumes. III. Auxin metabolism in effective and ineffective pea root nodules.Plant Physiol. 73, 347–352 (1983).

    PubMed  CAS  Google Scholar 

  • Badenoch-Jones J., Summons R.E., Rolfe B.G., Letham D.S.: Phytohormones,Rhizobium mutants, and nodulation in legumes. IV. Auxin metabolism in pea root nodules.J. Plant Growth Regul. 3, 23–39 (1984).

    Article  CAS  Google Scholar 

  • Bray H.G., Thorpe W.V.: Analysis of phenolic compounds of interest in metabolism, pp. 27–52 inMethods of Biochemical Analysis (D. Glick, Ed.). Interscience Publication, New York 1954.

    Chapter  Google Scholar 

  • Conn H.J., Jennison M.W., Weeks D.B.: Routine test for the identification of bacteria, pp. 140–168 inManual of Microbiological Methods (H.J. Conn, Ed.). McGraw-Hill Book Co., New York 1957.

    Google Scholar 

  • Datta C., Basu P.S.: Content of indole acetic acid and its metabolism in root nodules ofCajanus cajan (L.)Millsp.Plant Physiol. Biochem. 24, 71–74 (1997).

    Google Scholar 

  • De P.S., Basu P.S.: Content of hormones and indole acetic acid metabolism in root nodules ofTephrosia purpurea Pers.Indian J. Plant Physiol. 38, 228–232 (1995).

    CAS  Google Scholar 

  • Dullaart J.: Quantitative estimation of indole acetic acid in root nodules and roots ofLupinus luteus L..Acta Bot. Neerl. 16, 222–230 (1967).

    CAS  Google Scholar 

  • Dullaart J.: The auxin content of root nodules and roots ofAlnus glutinosa (L.)Vill.J. Exp. Bot. 21, 975–984 (1970).

    Article  CAS  Google Scholar 

  • Gordon S.A., Weber R.P.: Colorimetric estimation of indole acetic acid.Plant Physiol. 26, 192–195 (1951).

    PubMed  CAS  Google Scholar 

  • Hassan S.S.M.: Spectrophotometric method for simultaneous determination of tryptophan and tyrosine.Anal. Chem. 47, 1429–1432 (1975).

    Article  PubMed  CAS  Google Scholar 

  • Hunter W.J.: Indole-3-acetic acid production by bacteroids from soybean root nodules.Physiol. Plant. 76, 31–36 (1989).

    Article  CAS  Google Scholar 

  • Jordan D.C.:Rhizobiaceae, pp. 235–240 inBergey's Manual of Systematic Bacteriology (N.R. Kried, J.G. Holt, Eds). Williams and Wilkins Co., Baltimore (USA) 1984.

    Google Scholar 

  • Kar M., Mishra D.: Catalase, peroxidase and polyphenol oxidase activities during rice leaf senescence.Plant Physiol. 57, 315–319 (1976).

    PubMed  CAS  Google Scholar 

  • Kefford N.P., Brockwell J., Zwar J.A.: The systematic synthesis of auxin by legumes and nodule bacteria and its role in nodule development.Austral. J. Biol. Sci. 13, 456–467 (1960).

    CAS  Google Scholar 

  • Kokkinakis D.M., Brooks J.L.: Hydrogen peroxide mediated oxidation of indole-3-acetic acid by tomato peroxidase and molecular oxygen.Plant Physiol. 64, 220–223 (1979).

    Article  PubMed  CAS  Google Scholar 

  • Nitsch J.P.: Free auxin and free tryptophan in strawberry.Plant Physiol. 30, 33–39 (1955).

    PubMed  CAS  Google Scholar 

  • Nutman P.S.: Study frame works for symbiotic nitrogen fixation, pp. 443–447 inRecent Developments in Nitrogen Fixation (W. Newton, J.R. Postgate, C. Rodriguez-Barrueco, Eds). Academic Press, London 1977.

    Google Scholar 

  • Panse V.G., Sukhatme P.V.:Statistical Methods for Agricultural Workers, 4th ed., pp. 145–156. Indian Council of Agricultural Research, New Delhi (India) 1985.

    Google Scholar 

  • Sinha B.K., Basu P.S.: Indole-3-acetic acid and its metabolism in root nodules ofPongamia pinnata (L.)Pierre.Biochem. Physiol. Pflanzen 176, 218–227 (1981).

    CAS  Google Scholar 

  • Skerman V.B.D.:A Guide to Identification of Genera of Bacteria, pp. 189–191. Williams and Wilkins Co., Baltimore 1959.

    Google Scholar 

  • Stafford H.A.: The metabolism of aromatic compounds.Ann. Rev. Plant Physiol. 25, 459–486 (1974).

    Article  CAS  Google Scholar 

  • Vance C.P.: Comparative aspects of root and nodule secondary metabolism.Phytochemistry 17, 1889–1891 (1978).

    Article  CAS  Google Scholar 

  • Wheeler C.T., Henson I.E., Mc Laughlin M.E.: Hormones in plants bearing actinomycete nodules.Bot. Gaz. 140 (Suppl.) S52-S57 (1979).

    Article  Google Scholar 

  • Williams M.N.V., Singer E.R.: Metabolism of tryptophan and tryptophan analogs byRhizobium meliloti.Plant Physiol. 92, 1009–1013 (1990).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Datta, C., Basu, P.S. Content of indoleacetic acid and its metabolism in root nodules ofMelilotus alba . Folia Microbiol 43, 427–430 (1998). https://doi.org/10.1007/BF02818586

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02818586

Keywords

Navigation