Abstract
Effect of oxygen and metabolic substrates (glucose, ethanol) on the catalase activity of anaerobically grownSchizosaccharomyces pombe cells was assessed, and compared with that ofSaccharomyces cerevisiae in order to determine the catalase activity regulation inS. pombe. In contrast toS. cerevisiae, the total catalase activity of permeabilizedS. pombe anaerobically grown cells is higher than that found in aerobically grown cells, is stable and constant under all circumstances (i.e. it is not induced by oxygen and/or substrates), and only a negligible part (3–5%) of it is contributed byde novo protein synthesis during aeration with or without substrates. The patent catalase activity of intact cells rises 2-fold during 6-h aeration without substrate and 7–8-fold in the presence of glucose or ethanol. The increase is not inhibited by cycloheximide and is thus not due tode novo catalase synthesis, but may reflect enhanced transport of catalase to the cell surface or a permeabilization of the plasma membrane during the aeration.
Similar content being viewed by others
References
Barnett J.A., Payne R.W., Yarrow D.:Yeasts: Characteristics and Identification, 2nd ed., p. 611. Cambridge University Press, Cambridge 1990.
Berte C., Sels A.: Catalase anabolism in yeast: loss of regulation by oxygen of catalase apoprotein synthesis after mutation.Mol. Gen. Genet.172, 45–52 (1979).
Gille G., Sigler K., Höfer M.: Response of catalase activity and membrane fluidity of aerobically grownSchizosaccharomyces pombe andSaccharomyces cerevisiae to aeration and the presence of substrates.J. Gen. Microbiol.139, 1627–1634 (1993).
Gutz H., Heslot H., Leupold U., Loprieno N.:Schizosaccharomyces pombe, pp. 395–446 in R.C. King (Ed.):Handbook of Genetics, Vol. 1 Plenum Press, London 1974.
Hörtner H., Ammerer G., Hartter E., Hamilton B., Rytka J., Bilinski T., Ruis H.: Regulation of synthesis of catalases and iso-1-cytochromec inSaccharomyces cerevisiae by glucose, oxygen and heme.Eur. J. Biochem.128, 179–184 (1982).
Janda S., Gille G., Sigler K., Höfer M.: Effect of hydrogen peroxide on sugar transport inSchizosaccharomyces pombe. Absence of membrane lipid peroxidation.Folia Microbiol.38, 135–140 (1993).
Kaplan J.G.: The alteration of intracellular enzymes. II. The relation between the surface and the biological activities of altering agents.J. Gen. Physiol.38, 197–211 (1954).
Kaplan J.G.: Heat inactivation of cryptic catalase of yeast.Nature196, 950–953 (1962).
Komarova L.I.: Ethanol yields, sugar fermentation and cell proliferation in mixed yeast cultures (In Russian),Mikrobiologiya6, 1047 (1937).
Kudryavtsev V.I.:Yeast Systematics (in Russian), pp. 339–340. USSR Acad. Sci. Publishing House, Moscow 1954.
Mitchison J.M.: Physiological and cytological methods forSchizosaccharomyces pombe.Meth. Cell Physiol.4, 131–165 (1970).
Rottenbach E.: Über die dextrinvergährende HefeSchizosaccharomyces pombe und ihre eventuelle Einführung in der Praxis.Z. Spiritusindustrie 58 (1896); cited from V.I. Kudryavtsev:Yeast Systematics (in Russian), pp. 339–340. USSR Acad. Sci. Publishing House, Moscow 1954.
Sigler K., Knotková A., Kotyk A.: Factors governing substrate-induced generation and extrusion of protons in the yeastSaccharomyces cerevisiae.Biochim. Biophys. Acta643, 572–582 (1981).
Zhukov I.: Über den Säurenverbrauch der Hefe.Zbl. Bakteriol.2, 601, (1896a); cited from V.I. Kudryavtsev:Yeast Systematics (in Russian), pp. 339–340. USSR Acad. Sci. Publishing House, Moscow 1954.
Zhukov I.: Gähr- und Konkurenzversuche mit verschiedenen Hefen.Kochs Jahresbericht 104 (1896,b); cited from V.I. Kudryavtsev:Yeast Systematics (in Russian), pp. 339–340. USSR. Acad. Sci. Publishing House, Moscow 1954.
Zimniak P., Hartter E., Ruis H.: Biosynthesis of catalase T during oxygen adaptation ofSaccharomyces cerevisiae.FEBS Lett.59, 300–304 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sigler, K., Gille, G. Stability and refractoriness of the high catalase activity in the oxidative-stress-resistant fission yeastSchizosaccharomyces pombe . Folia Microbiol 43, 369–372 (1998). https://doi.org/10.1007/BF02818575
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02818575