Folia Microbiologica

, Volume 46, Issue 6, pp 565–572 | Cite as

The role of microflora in the development of intestinal inflammation: Acute and chronic colitis induced by dextran sulfate in germ-free and conventionally reared immunocompetent and immunodeficient mice

  • T. Hudcovic
  • R. Štěpánková
  • J. Cebra
  • H. Tlaskalová-Hogenová
Immunology Papers


One-week dextran sulfate treatment of conventional (CV) immunodeficient (SCID) mice gave rise to acute colitis in the colon mucosa; germ-free (GF) SCID mice did not exhibit any changes in colon morphology. Dextran sulfate application to CV immunocompetent (BALB/c) mice did induce substantial changes in the colon mucosa (grade4); GF BALB/c mice showed mild changes in the colon morphology (grade1) only. GF SCID mice and CV SCID mice died during the second round of dextran sulfate treatment suffering from chronic colitis; GF BALB/c mice exhibited mild crypt distortion while CV BALB/c mice showed a complete loss of the surface epithelium (grade4), accompanied by T and B lymphocyte infiltration.


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Copyright information

© Folia Microbiologica 2001

Authors and Affiliations

  • T. Hudcovic
    • 1
  • R. Štěpánková
    • 1
  • J. Cebra
    • 2
  • H. Tlaskalová-Hogenová
    • 2
  1. 1.Department of Immunology and Gnotobiology, Institute of MicrobiologyAcademy of Sciences of the Czech RepublicNový HrádekCzechia
  2. 2.Department of BiologyUniversity of PennsylvaniaPhiladelphiaUSA

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