Abstract
A DNA fragment from phage ΦU1 containing an origin of DNA replication was identified. This fragment, designatedori, was able to support the maintenance inStreptomyces lividans of a plasmid lacking a functional Gram-positiveori. The sequence of the minimalori fragment was determined and analyzed. The minimal fragment conferring replication origin function contained a number of direct and inverted repeats. The absence of an open reading frame in thisori fragment indicates that host factors alone were sufficient to initiate replication atori.
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Farkašovská, J., Godány, A., Bukovská, G. et al. An origin of DNA replication from streptomycete phage ΦU1. Folia Microbiol 46, 483–487 (2001). https://doi.org/10.1007/BF02817990
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DOI: https://doi.org/10.1007/BF02817990