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Rylux BSU stimulates spore germination inTrichophyton mentagrophytes andAspergillus fumigatus and increases the survival rate after UV-Irradiation

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Abstract

Calcofluor-allied optical brightener Rylux BSU stimulated spore germination rate inTrichophyton mentagrophytes andAspergillus fumigatus both if supplemented into Sabouraud glucose agar and if used for pretreatment of spore suspension prior to inoculation at low concentrations. Maximum stimulation of germination was obtained if 0.2% Rylux BSU was used for pretreatment in aqueous solution for 1 d prior to inoculation (130% inT. mentagrophytes and 150% inA. fumigatus, respectively). Pretreatment with 1% Rylux BSU provided strong protection against UV-irradiation and resulted in increased yields of cultural variants after UV-irradiation.

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References

  • Bartnicki-García S., Persson J., Chanzy H.: An electron microscope and electron diffraction study of the effect of Calcofluor and Congo red on the biosynthesis of chitinin vitro.Arch. Biochem. Biophys. 310, 6–15 (1994).

    Article  PubMed  Google Scholar 

  • Darken M.A., Swift M.E.: Effects of ultraviolet-absorbing compounds on spore germination and cultural variation in microorganisms.Appl. Microbiol. 11, 154–156 (1963).

    PubMed  CAS  Google Scholar 

  • Ghormade V.S., Lachke S.A., Deshpande M.V.: Dimorphism inBenjaminiella poitrasii: Involvement of intracellular endochitinase andN-acetylglucosaminidase in the yeast-mycelium transition.Folia Microbiol. 45, 231–238 (2000).

    Article  CAS  Google Scholar 

  • Haplová J., Farkaš V., Hejtmánek M., Koďousek R., Malínský J.: Effect of the new fluorescent brightener Rylux BSU on morphology and biosynthesis of cell walls inSaccharomyces cerevisiae.Arch. Microbiol. 161, 340–344 (1994).

    PubMed  Google Scholar 

  • Hartland R.P., Vermeulen C.A., Klis F.M., Sietsma J.H., Wessels J.G.: The linkage of (1–3)-β-glucan to chitin during cell-wall assembly inSaccharomyces cerevisiae.Yeast 10, 1591–1599 (1994).

    Article  PubMed  CAS  Google Scholar 

  • Hejtmánek M., Koďousek R., Hejtmánková N.: Fluorescent microscopic assay of mycopathogens using Rylux BSU. (In Czech)Českoslov. Pathol. 25, 244–250 (1989).

    Google Scholar 

  • Hejtmánek M., Doležel J., Holubová I.: Staining of fungal cell walls with fluorescent brighteners: flow-cytometric analysis.Folia Microbiol. 35, 437–442 (1990).

    Article  Google Scholar 

  • Hejtmánek M., Koďousek R., Raclavský V.: The fluorescence brightener Rylux BSU induces dimorphism inBasidiobolus ranarum.Folia Microbiol. 38, 395–398 (1993).

    Google Scholar 

  • Pedraza-Reyes M., Lopez-Romero E.: Chitinase activity in germinating cells ofMucor rouxii.Antonie van Leeuwenhoek 59, 183–189 (1991).

    PubMed  CAS  Google Scholar 

  • Raclavský V., Hejtmánek M.: Quantitative assessment of yeasts in sputum—direct microscopic method. (In Czech)Českoslov. Epidemiol. Mikrobiol. Imunol. 38, 161–166 (1989).

    Google Scholar 

  • Raclavský V., Kopeček P.: Effect of the fluorescent brightener Rylux BSU on the cell wall chitin content inBasidiobolus ranarum.Acta Univ. Palacki Olomuc. Fac. Med. 138, 19–20 (1994).

    PubMed  Google Scholar 

  • Raclavský V., Novotný R., Šmígová J., Vojküvka Z.: Nikkomycin Z counteracts Rylux BSU and Congo red inhibition ofSaccharomyces cerevisiae growth but does not prevent formation of aberrant cell walls.Folia Microbiol. 44, 663–668 (1999).

    Article  Google Scholar 

  • Takaya N., Yamazaki D., Horiuchi H., Ohta A., Takagi M.: Cloning and characterization of a chitinase-encoding gene (chiA) fromAspergillus nidulans, disruption of which decreases germination frequency and hyphal growth.Biosci. Biotechnol. Biochem. 62, 60–65 (1988).

    Article  Google Scholar 

  • Vermeulen C.A., Wessels J.G.: Chitin biosynthesis by a fungal membrane preparation. Evidence for a transient non-crystalline state of chitin.Eur. J. Biochem. 158, 411–415 (1986).

    Article  PubMed  CAS  Google Scholar 

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Chalupová, V., Raclavský, V. & Novotný, R. Rylux BSU stimulates spore germination inTrichophyton mentagrophytes andAspergillus fumigatus and increases the survival rate after UV-Irradiation. Folia Microbiol 47, 152–156 (2002). https://doi.org/10.1007/BF02817673

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  • DOI: https://doi.org/10.1007/BF02817673

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