Abstract
In shaken cultures ofPhanerochœte chrysosporium, different Tweens gave rise to similar and high lignin peroxidase (LiP) activities. The polyoxyethylene-sorbitan (POE-S) moieties isolated from Tweens gave rise to somewhat lower LiP activities, whereas fatty acids isolated from Tweens gave rise to much lower LiP activities than parent Tweens. LiP activity appeared 3 d after addition of Tween 80 if this was added within the first 4 d after inoculation. Of the three chemical moieties contained in Tweens,i.e., fatty acids, sorbitan, and polyoxyethylene (POE), only the latter one significantly stimulated the LiP activity of the culture. The stimulatory effect of POE on the LiP activity increased till its molar mass of approx. 1 kDa, then it levelled off. The quantity of POE in the culture decreased with time. Tween 80, its POE-S moiety and POEs seem to enhance LiP production and not only their release.
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Asther M., Corrieu G., Drapron R., Odier E.: Production of ligninases and degradation of lignin in agitated submerged cultures ofPhanerochœte chrysosporium.Enzyme Microb. Technol. 9, 245–249 (1987).
Asther M., Lesage L., Drapron R., Corrieu G., Odier E.: Phospholipid and fatty acid enrichment ofPhanerochœte chrysosporium INA-12 in relation to ligninase production.Appl. Microbiol. Biotechnol. 27, 393–398 (1988).
Bumpus J.A., Tien M., Wright D., Aust S.D.: Oxidation of persistent pollutants by a white rot fungus.Science 228, 1434–1436 (1985).
Capdevila C., Moukha S., Ghyczy M., Theilleux J., Gelie B., Delattre M., Corrieu G., Asther M.: Characterization of peroxidase secretion and subcellular organization ofPhanerochœte chrysosporium INA-12 in the presence of various soybean phospholipid fractions.Appl. Environ. Microbiol. 56, 3811–3816 (1990).
Jäger A., Croan S., Kirk T.K.: Production of ligninases and degradation of lignin in agitated submerged cultures ofPhanerochœte chrysosporium.Appl. Environ. Microbiol. 50, 1274–1278 (1985).
Kawai S., Jensen K.A., Bao W., Hammel K.E.: New polymeric model substrates for the study of microbial ligninolysis.Appl. Environ. Microbiol. 61, 3407–3414 (1995).
Kirk T.K., Farrell R.L.: Enzymatic “combustion”: the microbial degradation of lignin.Ann. Rev. Microbiol. 41, 465–505 (1987).
Leštan D., Černilec M., Štrancar A., Perdih A.: Influence of some surfactants and related compounds on ligninolytic activity ofPhanerochœte chrysosporium.FEMS Microbiol. Lett. 106, 17–22 (1993).
Leštan D., Leštan M., Perdih A.: Physiological aspects of biosynthesis of lignin peroxidases byPhanerochœte chrysosporium.Appl. Environ. Microbiol. 60, 606–612 (1994).
Park J.W., Takahata Y., Kajiuchi T., Akehata T.: Effects of nonionic surfactant on enzymatic hydrolysis of used newspaper.Biotechnol. Bioeng. 39, 117–120 (1992).
Perdih A., Leštan D.: The role of surfactants in enhancement of ligninolytic activity inPhanerochœte chrysosporium.Acta Chim. Slov. 40, 29–35 (1993).
Podgornik H., Podgornik A., Perdih A.: Kinetic measurements of lignin peroxidase activity.Acta Chim. Slov. 44, 253–260 (1997).
Tien M., Kirk T.K.: Lignin degrading enzyme fromPhaneroehœte chrysosporium: purification, characterization and catalytic properties of a unique H2O2-requiring oxygenase.Proc. Nat. Acad. Sci. USA 81, 2280–2284 (1984).
Tomaževič L., Perdih A.: Determination of intrapellet ligninolytic activity.Folia Microbiol. 41, 499–501 (1996).
Touchstone J.C., Dobbins M.F.:Practice of Thin Layer Chromatography, pp. 170, 189. J. Wiley & Sons, New York 1978.
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Katič, M., Frantar, J., Grgič, I. et al. Polyoxyethylene stimulates lignin peroxidase production inPhanerochœte chrysosporium . Folia Microbiol 43, 631–634 (1998). https://doi.org/10.1007/BF02816381
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DOI: https://doi.org/10.1007/BF02816381