Abstract
Synthetic and natural zeolite were used to control the nitrogen source level inBacillus megaterium cultures producing extracellular proteinase by means of reversible adsorption. The addition of biochemically inert, calcium aluminosilicates significantly increased the yield of the enzyme. A simple mathematical model explaining the mechanism has been formulated and its parameters estimated.
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Castellar M.R., Aires-Barros M.R., Cabral J.M.S.: Effect of zeolite on ethanol fermentation, inProc. FEBS Internat. Symp. Immobilized Cells: Basic and Applications (R.H. Wijffels, R.M. Buitelaar, H.G. Westels, C. Buckl, J. Tramper, Eds). Noordwijkerhont, The Netherlands 1995.
Chaloupka J., Obdržálek V., Křečková P. Nesmeyanova M.A., Zalabák V.: Protease activity in cellsBacillus megaterium during depression.Folia Microbiol. 20, 277–288 (1975).
Luebbe C., Demain A.L., Bergman K.: Use of controlled-release polymer to feed ammonium toStreptomyces clavuligerus cephalosporin fermentations in shake flask.Appl. Microbiol. Biotechnol. 22, 424–427 (1985).
Ma T.S., Zuazaga G.: Micro-Kjeldahl determination of nitrogen. A new indicator and improvement of method.Ind. Eng. Chem. Anal. 14, 280–286 (1942).
Markham R.: A steam distillation apparatus suitable for micro-Kjeldahl analysis.Biochem. J. 36, 790–791 (1942).
Millet J., Aubert J.P.: Etude de la megaterispeptidase, protease exocellulaire deBacillus megaterium. III. Biosynthese et de role physiologique.Ann. Inst. Pasteur 117, 461–473 (1969).
Moravcová J., Chaloupka J.: Repression of the synthesis of exocellular and intracellular proteinases inBacillus megaterium Folia Microbiol. 29, 273–281 (1984).
Nayve F.R., Motoki M., Matsumura M., Kataoka H.: Selective removal of ammonia from animal cell culture broth.Cytotechnology 6, 121–130 (1991).
Omura S., Tanaka Y., Takahashi Y., Iwai Y.: Stimulation of the production of macrolide antibiotics by magnesium phosphate and related insoluble materials.J. Antib. 33, 1568–1569 (1980).
Pazlarová J., Votruba J.: Use of zeolite to control ammonium inBacillus amyloliquefaciens α-amylase fermentations.Appl. Microbiol. Biotechnol. 45, 315–318 (1996).
Pirt S.J.:Principles of Microbe and Cell Cultivation. Blacwell, Oxford 1975.
Tchelishtchev N.F., Berenshtein B.G., Volodin V.F.:Zeolites—New Mineral Raw Material. (In Russian) Publ. House Nedra, Moscow 1987.
Votruba J., Kůtová A., Machová A., Sobotka M., Pazlarová J.: Fermentation method of preparation of hydrolytic exocellular enzymes.Czech Pat. 278 974 (1994).
Votruba J., Pazlarová J., Dvořáková M., Váchová L., Strnadová M., Kučerová H., Vinter V., Zoubarian R., Chaloupka J.: External factors involved in the regulation of an extracellular proteinase synthesis inBacillus megaterium. The effect of glucose and amino acids.Appl. Microbiol. Biotechnol. 26, 373–377 (1987).
Witter E., Lopez-Real J.: Nitrogen losses during the composting of sewage sludge, and the effectiveness of clay soil, zeolite and compost in adsorbing the volatalize ammonia.Biol. Wastes 23, 279–294 (1988).
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Votruba, J., Pazlarová, J. & Chaloupka, J. Use of zeolite to controlBacillus megaterium extracellular proteinase production. Folia Microbiol 43, 613–616 (1998). https://doi.org/10.1007/BF02816377
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DOI: https://doi.org/10.1007/BF02816377