Abstract
We have used the T7 expression system for expression ofE. coli FNR protein. Thefnr gene was cloned from its initiation codon ATG into theNdeI site of an expression vector and filamentous phage mGP1-2 was used as a donor of T7 RNA polymerase gene. The level of FNR expression attained by this expression arrangement was about 45% of total cell proteins.
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Stuchlík, S., Turňa, J. Overexpression of the FNR protein ofEscherichia coli with T7 expression system. Folia Microbiol 43, 601–604 (1998). https://doi.org/10.1007/BF02816375
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DOI: https://doi.org/10.1007/BF02816375