Abstract
Protein kinases can be classified into two main superfamilies on the basis of their sequence similarity and substrate specificity. The protein His kinase superfamily which autophosphorylate a His residue, and superfamily Ser/Thr and Tyr protein kinases, which phosphorylate Ser, Thr or Tyr residues. During the last years genes encoding Ser/Thr protein kinases have been identified in several microorganisms. Phosphorylation of proteins on Ser/Thr residues can be involved in many functions of prokaryotic cells including cell differentiation, signal transduction and protein biosynthesis. Phosphorylation of prokaryotic protein-synthesizing systems showed that the phosphorylation of initiation and elongation factors is subject to alteration during cell differentiation or bacteriophage infection. Protein kinase associated with ribosomes of streptomycetes phosphorylate the elongation factor Tu and 11 ribosomal proteins even in bacteriophage-uninfected cells. After phosphorylation of ribosomal proteins, ribosomes lose about 30% of their activity at the translation of poly(U).
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Alexander C., Bilgin N., Lindschau C., Mesters J.R., Kraal B., Hilgenfeld R., Erdmann V.A., Lipman C.: Phosphorylation of elongation factor Tu prevents ternary complex formation.J. Biol. Chem. 270, 14541–14547 (1995).
Fakunding J.L., Traugh J.A., Traut R.R., Hershey J.W.B.: Phosphorylation of initiation factor IF 2 fromEscherichia coli with skeletal muscle kinase.J. Biol. Chem. 247, 6365–6367 (1972).
Geyl D., Bock A., Isono K.: An improved method for two-dimensional gel-electrophoresis: Analysis of mutationally altered ribosomal proteins ofEscherichia coli.Mol Gen. Genet. 181, 309–312 (1981).
Hanlon W.A., Inouye M., Inouye S.: Pkn9, a Ser/Thr protein kinase involved in the development ofMyxococcus xanthus.Mol. Microbiol. 23, 459–471 (1997).
Hong S.-K., Matsumoto A., Horinouchi S., Beppu T.: Effect of protein kinase inhibitors onin vitro protein phosphorylation and cellular differentiation ofStreptomyces griseus.Mol. Gen. Genet. 236, 347–354 (1993).
Kobayashi H., Kobayashi K., Kobayashi Y.: Isolation and characterization of fusidic acid-resistant sporulation-defective mutants ofBacillus subtilis.J. Bacteriol. 132, 262–269 (1977).
Lippmann C., Lindschau C., Vijgenboom E., Schroder W., Bosch L., Erdmann V.A.: Prokaryotic elongation factor Tu is phosphorylatedin vivo.J. Biol. Chem. 268, 601–607 (1993).
Mikulík K., Zhoulanova L.: Sequencing of thetufl gene and the phosphorylation pattern of EF-Tu1 during development and differentiation ofStreptomyces collinus.Biochem. Biophys. Res. Commun. 213, 454–461 (1995).
Mikulík K., Dobrová Z., Janeček J., Quoc Hoang Khanh: Pattern of phosphoproteins during cell differentiation inStreptomyces collinus.FEMS Microbiol. Lett. 158, 147–151 (1998).
Mitchell C., Morris P.W., Vary C.J.: Identification of proteins phosphorylated by ATP during sporulation ofBacillus subtilis.J. Bacteriol. 174, 2474–2477 (1992).
Pearson W.R., Lipman D.J.: Improved tools for biological sequence comparison.Proc. Nat. Acad. Sci. USA 85, 2444–2448 (1988).
Robertson E.S., Nicholson A.W.: Protein kinase of bacteriophage T7 induces the phosphorylation of only a small number proteins in the infected cells.Virology 175, 525–534 (1990).
Robertson E.S., Nicholson A.W.: Phosphorylation ofEscherichia coli translation initiation factors by the bacteriophage T7 protein kinase.Biochemistry 31, 4822–4827 (1992).
Robertson E.S., Aggison L.A., Nicholson A.W.: Phosphorylation of elongation factor G and ribosomal protein S6 in bacteriophage T7-infectedEscherichia coli.Mol. Microbiol. 11, 1045–1057 (1994).
Traugh J.A., Traut R.R.: Phosphorylation of ribosomal proteins ofEscherichia coli by protein kinase from skeletal muscle.Biochemistry 11, 2501–2508 (1972).
Vijgenboom E., Woudt L.P., Heinstra P.W.H., Rietveld K., Haarlem J., Wezel G.P., Shochat S., Bosch L.: Threetuf-like gene in the kirromycin producerStreptomyces ramocissimus.Microbiology 140, 983–998 (1994).
van Wezel P.G., Woudt L.P., Vervenne R., Verdurmen M.L.A., Vijgenboom E., Bosch L.: Cloning and sequencing of thetuf genes ofStreptomyces coelicolor A3(2).Biochim. Biophys. Acta 1219, 543–547 (1994).
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Mikulík, K., Zhoulanova, E., Hoang, QK. et al. Protein kinase associated with ribosomes of streptomycetes. Folia Microbiol 44, 123–130 (1999). https://doi.org/10.1007/BF02816231
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DOI: https://doi.org/10.1007/BF02816231