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Growth and cyclosporin a production by an indigenously isolated strain ofTolypocladium inflatum

Folia Microbiologica volume 41, pages 401–406 (1996)Cite this article

Abstract

A fungal culture isolated from a local soil sample which showed antifungal activity and produced cyclosporin A, was identified asTolypocladium inflatum. The culture grew best in a medium containing 1% maltose (pH 5–6) when inoculated with a one-day-old inoculum at 2% (V/V) concentration. Under batch fermentation conditions, growth and cyclosporin A production were better in complex media (24.6 g biomass and 205 mg cyclosporin A per liter) in comparison with synthetic media (6.84 g biomass and 35 mg cyclosporin A per liter). While addition of peptone increased culture growth (high biomass yield), supplementation with casein acid hydrolyzate favored cyclosporin A production.

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Affiliations

  1. Biotechnology Unit, Regional Research Laboratory, Council of Scientific & Industrial Research, 695 019, Trivandrum, India

    K. Balakrishnan & Ashok Pandey

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  1. K. Balakrishnan
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  2. Ashok Pandey
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Balakrishnan, K., Pandey, A. Growth and cyclosporin a production by an indigenously isolated strain ofTolypocladium inflatum . Folia Microbiol 41, 401–406 (1996). https://doi.org/10.1007/BF02815689

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  • DOI: https://doi.org/10.1007/BF02815689

Keywords

  • Cyclosporin
  • Maltose
  • Antifungal Activity
  • Ergot Alkaloid
  • Microbial Metabolite