Abstract
The mechanism of pathogenicity of 13Aeromonas caviae strains isolated from fecal specimens of children with diarrhea was examined. Isolates possessed several virulence agents. Six of 13 isolates produced cholera-like toxin, all strains exhibited adhesive ability and secreted catecholate siderophores. Six isolates were able to grow in heat-inactivated serum. Production of hemolysins was not detected. The isolates had relatively high values of LD50 in a mouse model, which suggests that there is no linkage between pathogenicity to mice and the diarrheagenic ability of the isolates.
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References
Altwegg M.: Aeromonas caviae: an enteric pathogen.Infection 13, 228–230 (1985).
Altwegg M., Geiss H.K.:Aeromonas as a human pathogen.CRC Crit. Rev. Microbiol. 16, 253–286 (1989).
Arnow L.E.: Colorimetric determination of the component of 3,4-dihydroxyphenylalanine-tyrosine mixtures.J. Biol. Chem. 118, 531–541 (1937).
Araujo R.M., Arribas R.M., Pares R.: Distribution ofAeromonas species in waters with different levels of pollution.J. Appl. Bacteriol. 71, 182–186 (1991).
Asao T., Kozaki S., Kato K., Kinoshita Y., Otsu K., Uemura T., Sakaguchi G.: Purification and characterization of anAeromonas hydrophila hemolysin.J. Clin. Microbiol. 24, 228–232 (1986).
Barer M.R., Millership S.E., Tobaqchali S.: Relationship of toxin production to species in the genusAeromonas.J. Med. Microbiol. 22, 303–309 (1986).
Burke V., Robinson J., Atkinson H.M., Dibley M., Berry R.J., Gracey M.: Exotoxins ofAeromonas hydrophila.Austral. J. Exp. Biol. Med. Sci. 59, 753–761 (1981).
Byers B.R., Arceneaux J.E.L.: Siderophore diversity in the genusAeromonas.Med. Microbiol. Lett. 2, 281–285 (1993).
Chopra A.K., Houston C.W.: Purification and partial characterization of a cytotonic enterotoxin produced byAeromonas hydrophila.Can. J. Microbiol. 35, 719–727 (1989).
Csaky T.Z.: On the estimation of bound hydroxylamine in biological materials.Acta Chem. Scand. 2, 450–454 (1948).
Daily O.P., Joseph S.W., Coolbaugh J.C., Walker R.I., Merrell B.R., Rollins D.M., Seidler R.J., Colwell R.R., Lissner C.R.: Association ofAeromonas sobria with human infection.J. Clin. Microbiol. 13, 769–777 (1981).
Deodhar L.P., Saraswathi K., Varudkar A.:Aeromonas spp. and their association with human diarrhoel disease.J. Clin. Microbiol. 29, 853–856 (1991).
Esteve C., Amaro C., Garay E., Santos Y., Toranzo A.E.: Pathogenicity of live bacteria and extracellular products of motileAeromonas isolated from eels.J. Appl. Bacteriol. 78, 555–562 (1995).
Gosling P.J., Turnbull P.C.B., Lightfoot N.F., Pether J.V.S., Lewis R.J.: Phenotypic characteristics ofAeromonas species as markers of enterotoxigenicity.Med. Microbiol. Lett. 2, 287–295 (1993).
Hänninen M.L., Salmi S., Mattila L., Taipalinen R., Siitonen A.: Association ofAeromonas spp. with travellers diarrhoea in Finland.J. Med. Microbiol. 42, 26–31 (1995).
Holmberg S.D., Schell W.L., Fanning G.R., Wachsmuth J.K., Hickmann-Brenner F.W., Blake P.A., Brenner D.J., Farmer J.J. III:Aeromonas intestinal infections in the United States.Ann. Intern. Med. 105, 683–689 (1986).
Janda J.M., Reitano M., Bottone E.B.: Biotyping ofAeromonas isolates as a correlation to delineating a species—associated disease spectrum.J. Clin. Microbiol. 19, 44–47 (1984).
Janda J.M., Clark R.B., Brenden R.: Virulence ofAeromonas species as assessed through mouse lethality studies.Curr. Microbiol. 12, 163–168 (1985).
Janda J.M., Kokka R.P.: The pathogenicity ofAeromonas strains relative to genospecies and phenospecies identification.FEMS Microbiol. Lett. 90, 29–34 (1991).
Janda J.M., Guthertz L.S., Kokka R.P., Shimada T.:Aeromonas species in septicemia—laboratory characteristics and clinical observations.Clin. Infect. 19, 77–83 (1994).
Janda J.M., Abbott S.L.: Evolving concepts regarding the genusAeromonas: an expanding panorama of species, disease presentation, and unanswered questions.Clin. Infect. Dis. 27, 332–344 (1998).
Joseph S.W.:Aeromonas gastrointestinal disease: a case study in causation, pp. 311–335 in B. Austin, M. Altwegg, P.J. Gosling, S. Joseph (Eds):The Genus Aeromonas. John Wiley & Sons, Chichester (UK) 1996.
Kaznowski A.: Genomic species ofAeromonas strains isolated from children's diarrhoeal stools.Med. Microb. Lett. 4, 255–262 (1995).
Kuijper E.J., Steigerwalt A.G., Schoenmakers B.C.I.M., Peeters M.F., Zanen H.C., Brenner D.J.: Phenotypic characterization and DNA relatedness in human fecal isolates ofAeromonas spp.J. Clin. Microbiol. 27, 1531–1537 (1989).
Ljungh A., Eneroth P., Wadström T.: Cytotonic enterotoxin fromAeromonas hydrophila.Toxicon 20, 787–794 (1982).
Massad G.L., Arceneaux J.E.L., Byers B.R.: Acquisition of iron from host sources by mesophilicAeromonas species.J. Gen. Microbiol. 137, 237–241 (1991).
Mateos D., Anguita J., Naharro G., Paniagua C.: Influence of growth temperature on the production of extracellular virulence factors and pathogenicity of environmental and human strains ofAeromonas hydrophila.J. Appl. Bacteriol. 74, 111–118 (1993).
Millership S.E., Barer M.R., Tobaqchali S.: Toxin production byAeromonas spp. from different sources.J. Med. Microbiol. 22, 311–314 (1986).
Munoz P., Fernandez-Baca V., Pelaez T., Sanches R., Rodriguez-Creixems M., Bouza E.:Aeromonas peritonitis.Clin. Infect. Dis. 18, 32–37 (1994).
Nakasone N., Iwanaga M.: Quantitative evaluation of colonizing ability ofVibrio cholerae O1.Microbiol. Immunol. 31, 753–761 (1987).
Namdari H., Bottone E.J.: Microbiologic and clinical evidence supporting the role ofAeromonas caviae as a pediatric enteric pathogen.J. Clin. Microbiol. 28, 837–840 (1990).
Pazzaglia G., Sack R.B., Salzar E., Chea E., Leon-Barula R., Guerrero C.E., Palomino J.: High frequency of coinfecting enteropathogens inAeromonas-associated diarrhea of hospitalized Peruvian infants.J. Clin. Microbiol. 29, 1151–1156 (1991).
Potomski J., Burke V., Robinson J., Fumarola D., Miragliotta G.:Aeromonas cytonic enterotoxin cross-reactive with cholera toxin.J. Med. Microbiol. 23, 179–186 (1987).
Rautelin H., Hänninen M.L., Sivonen A., Turunen U., Valtonen V.: Chronic diarrhoea due to single strain ofAeromonas caviae.Eur. J. Clin. Microbiol. Infect. Dis. 14, 51–53 (1995).
Reed L.J., Muench H.: A simple method of estimating fifty per cent endpoints.Am. J. Hyg. 27, 493–497 (1937).
Schultz A., McCardell B.A.: DNA homology and immunological cross-reactivity betweenAeromonas hydrophila cytotonic toxin and cholera toxin.J. Clin. Microbiol. 26, 57–61 (1988).
Schwyn B., Neilands J.B.: Universal chemical assay for the detection and determination of siderophores.Anal. Biochem. 160, 47–56 (1987).
Singh D.V., Sanyal S.C.: Production of hemolysins and its correlation with enterotoxicity inAeromonas spp.J. Med. Microbiol. 31, 262–267 (1992).
Thornley J.P., Shaw J.G., Gryllos A., Eley A.: Virulence properties of clinically significantAeromonas species: evidence for pathogenicity.Rev. Med. Microbiol. 8, 61–72 (1997).
Wang G., Tyler K. D., Munro C. K., Johnson M. W.: Characterization of cytotoxic, hemolyticAeromonas caviae clinical isolates and their identification by determining presence of a unique hemolysin gene.J. Clin. Microbiol. 34, 3203–3205 (1996).
Yamamoto T., Yokota T.:Vibrio cholerae non-O1: production of cell-associated hemagglutinins andin vitro adherence to mucus coat and epithelial surfaces of the villi and lymphoid follicles of human small intestines treated with formalin.J. Clin. Microbiol. 26, 2018–2024 (1988).
Zywno S.R., Arceneaux J.E.L., Altwegg M., Byers B.R.: Siderophore production and DNA hybridization groups ofAeromonas spp.J. Clin. Microbiol. 30, 619–622 (1992).
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Mokracka, J., Krzyminska, S. & Szczuka, E. Virulence factors of clinical isolates ofAeromonas caviae . Folia Microbiol 46, 321–326 (2001). https://doi.org/10.1007/BF02815621
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DOI: https://doi.org/10.1007/BF02815621