Abstract
The influence of the postantibiotic effect (PAE) and the postantibiotic sub-MICs effect (PA SME) of imipenem and enoxacin on the surface hydrophobicity ofS. typhimurium andS. enteritidis strains were studied by evaluating Congo red binding and the aggregation in molar solutions of ammonium sulfate (SAT). A PAE was induced by 2× and 4× MIC of antibiotics tested for 0.5 h. Suprainhibitory concentrations of imipenem againstS. typhimurium induced a short PAE (0.3–0.6 h) compared toS. enteritidis (6.0–9.7 h). Suprasubinhibitory concentrations of imipenem did not allow a regrowth ofS. enteritidis. Similar results were also found for enoxacin. Evaluation of surface hydrophobic properties of the salmonellas after affecting both PAEs and PA SMEs has shown that imipenem at concentrations 4×MIC and 4×MIC+0.3×MIC partially influenced the hydrophobicity ofS. typhimurium. S. enteritidis was more susceptible toward both antibiotics tested.
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Blanco J., Alonso M.P., Gonzales E.A., Blanco M., Garabal J.I.: Virulence factors of bacteræmicEscherichia coli with particular reference to production of necrotising factor (CNF) by P-fimbriate strains.J. Med. Microbiol. 42, 175–183 (1990).
Bustamante C.J., Drusano G.Z., Tatem B.A., Standiford H.C.: Postantibiotic effects of imipenem onPseudomonas aeruginosa.Antimicrob. Agents Chemother. 26, 678–682 (1984).
Craig W.A., Gudmundsson S.: The postantibiotic effect, pp. 515–536 in V. Lorian (Ed.):Antibiotics in Laboratory Medicine, 2nd edn. Williams and Wilkins, Baltimore 1986.
Craig W.A., Ebert S.: Killing and regrowth of bacteriain vitro: a review.Scand. J. Infect. Dis. (Suppl.)74, 63–70 (1991).
Craig W.A., Gudmundsson S.: The postantibiotic effect, pp. 403–431 in V. Lorian (Ed.):Antibiotics in Laboratory Medicine, 3rd edn. Williams and Wilkins, Baltimore 1991.
Fang W.: A novel fluorometric method for evaluation of the postantibiotic effect of antimicrobial drugs on mastitis-causingStaphylococcus aureus andEscherichia coli.J. Microbiol. Meth. 26, 151–159 (1996).
Fuentes F., Martin M.M., Izquierdo J., Gomezlus M.Z., Prieto J.: Pharmacodynamic effects of ciprofloxacin, fleroxacin and lomefloxacinin vivo andin vitro.Chemotherapy 42, 354–362 (1996).
Gerceker A.A., Ötük G.: Postantibiotic effect on imipenem alone and in combination with amikacin onPseudomonas aeruginosa.Chemotherapy 41, 433–436 (1995).
Gottferdsson M., Erlendsdóttir H., Kolka R., Gudmundsson A., gudmundsson S.: Ultrastructural alterations of bacteria during the postantibiotic effect.Chemotherapy,39, 153–162 (1993).
Gould I.M., Jason A.C., Milne K.: Use of the Malthus microbial growth analyser to study the postantibiotic effect of antibiotics.J. Antimicrob. Chemother. 24, 523–531 (1989).
Guan L., Burnham J.C.: Postantibiotic effect of CI-960, enoxacin and ciprofloxacin onEscherichia coli effect on morphology and hæmolysin activity.J. Antimicrob. Chemother. 29, 529–538 (1992).
Gudmundsson S., Vogelman B., Craig W.A.: Decreased bactericidal activity during the period of the postantibiotic effect.J. Antimicrob. Chemother. 34, 921–930 (1994).
Löwdin E., Odenholt-Tornqvist I., Bengtsson S., Cars O.: A new method to determine postantibiotic effect and effects of subinhibitory antibiotic concentrations.Antimicrob. Agents Chemother. 37, 2200–2205 (1993).
Majtán V., Hybenová D.: Inhibition ofPseudomonas aeruginosa alginate expression by subinhibitory antibiotic concentrations.Folia Microbiol. 41, 61–64 (1996).
Meng X., Nightingale C.A., Sweeney K.R., Quintilani R.: Loss of bactericidal activities of quinolones during the postantibiotic effect induced by rifampicin.J. Antimicrob. Chemother. 33, 721–728 (1994).
Minguez F., Ramos C., Barrientos S., Loscos A., Prieto J.: Postantibiotic effect of ciprofloxacin compared with that of five other quinolones.Chemotherapy 37, 420–425 (1991).
Nadler H.L., Sheikh W.: A comparison of thein vitro postantibiotic effect of meropenem, and imipenemversus selectedEnterobacteriaceae and other pathogens.Diagn. Microbiol. Infect. Dis. 17, 71–73 (1993).
Odenholt-Tornqvist I.: Studies on the postantibiotic effect and the postantibiotic sub-MIC effect of meropenem.J. Antimicrob. Chemother. 31, 881–892 (1993).
Qadri F., Abu Hossain S., Čižnár I., Haider K., Ljungh A., Wadström T., Sack D.A.: Congo red binding and salt aggregation as indicators of virulence inShigella species.J. Clin. Microbiol. 26, 1343–1348 (1988).
Renneberg J., Walder M.: Postantibiotic effect of imipenem, norfloxacin and amikacinin vitro andin vivo.Antimicrob. Agents Chemother. 33, 1714–1720 (1989).
Sanford L.D., Jones R.N.: Post-antibiotic effects of E-4868 and OPC-17116.J. Antimicrob. Chemother. 32, 916–917 (1993).
Zhanel G.G., Karlowsky J.A., Davidson R.J., Hoban D.J.: Effect of pooled human cerebrospinal fluid on the postantibiotic effects of cefotaxime, ciprofloxacin and gentamicin againstEscherichia coli.Antimicrob. Agents Chemother. 36, 1136–1139 (1992).
Zhanel G.G., Crampton J., Kim S., Nicolle L.E., Davidson R.J., Hoban D.J.: Antimicrobial activity of subinhibitory concentrations of ciprofloxacin againstPseudomonas aeruginosa as determined by the killing curve method and the postantibiotic effect.Chemotherapy 38, 388–394 (1992).
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Majtánová, L., Majtán, V. Postantibiotic effects of imipenem and enoxacin againstS. typhimurium andS. enteritidis and the influence on their surface hydrophobicity. Folia Microbiol 43, 104–108 (1998). https://doi.org/10.1007/BF02815553
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DOI: https://doi.org/10.1007/BF02815553