Skip to main content

Influence of alkali-treated cornsteep liquor containing medium on protein A production byStaphylococcus aureus

Folia Microbiologica volume 43, pages 31–34 (1998)Cite this article

Abstract

Staphylococcus aureus cultivated in liquid media containing untreated cornsteep liquor (CSL) and alkali-treated CSL produced similar biomass yields (6.5–6.9 g/L). However, contents of protein A in the biomass was 0.5% and 1.56% for untreated CSL and treated CSL, respectively. Addition of treated CSL at 20 g/L achieved optimal enhancement of protein A production (0.11 g/L). Probable factors associated in treated CSL for the enhanced protein A production are discussed.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  • Arvidson S., Holme T., Wadström T.: Formation of bacteriolytic enzymes in batch and continuous culture ofStaphylococcus aureus.J. Bacteriol.104, 227–233 (1970).

    PubMed  CAS  Google Scholar 

  • Catalona W.J., Ratliff T.L., McCool R.E.: γ-Interferon induced byStaphylococcus aureus Protein A augments natural killing and ADCC.Nature291, 77–79 (1981).

    PubMed  Article  CAS  Google Scholar 

  • Clark S.J.:Quantitative Methods of Organic Microanalysis. Butterworths Sci. Publ., Oxford 1956.

    Google Scholar 

  • Fiedler F., Glaser X.Y.: Assembly of bacterial cell wall.Biochem. Biophys. Acta300, 467–485 (1973).

    CAS  Google Scholar 

  • Freiburghaus C., Ohlson S.V., Nilsson I.M.: Immobilised enzymes and cells.Methods Enzymol.137, 458–466 (1988).

    PubMed  CAS  Article  Google Scholar 

  • Grov A., Rude S.: Immunochemical characterization ofStaphylococcus aureus cell walls.Acta Pathol. Microbiol. Scand.71, 409–416 (1967).

    CAS  Article  Google Scholar 

  • Hjelm H., Hjelm K., Sjöquist J.: Protein A fromStaphylococcus aureus: Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins.FEBS Lett.28, 73–76 (1972).

    PubMed  Article  CAS  Google Scholar 

  • Kasparian S.S., Menezes J.: CD8+ suppressor cells inhibit staphylococcal Protein A induced γ-interferon.Immunol. Lett.27, 31 (1991).

    PubMed  Article  CAS  Google Scholar 

  • Landwall P.: Dialysis culture for the production of extracellular Protein A fromStaphylococcus aureus A676.J. Appl. Bacteriol.44, 151–158 (1978).

    PubMed  CAS  Google Scholar 

  • Lindemann R.A., Singh K.P., Shau H., Gupta R.K.: Effect of staphylococcal Protein A on human lymphokine activated killer cells.Cancer Immunol. Immunother.33, 97 (1991).

    PubMed  Article  CAS  Google Scholar 

  • Lindmark R., Movitz J., Sjöquist J.: Extracellular Protein A from a methicillin resistant strain ofStaphylococcus aureus.Eur. J. Biochem.74, 623–628 (1977).

    PubMed  Article  CAS  Google Scholar 

  • Lindmark R., Thoren-Tolling K., Sjöquist J.: Binding of immunoglobulins to Protein A and immunoglobulin levels in mammalian sera.J. Immunol. Meth.62, 1–13 (1983).

    Article  CAS  Google Scholar 

  • Movitz J.: Formation of extracellular Protein A byStaphylococcus aureus.Eur. J. Biochem.68, 291–299 (1976).

    PubMed  Article  CAS  Google Scholar 

  • Muniasamy N., Bhopale G.M., Ambedkar S.S., Naik S.R.: Protein A quantitation by competitive ELISA using penicillinase.Biotechnol. Tech.10, 779–782 (1996).

    Article  CAS  Google Scholar 

  • Siggia S.:Quantitative Organic Analysis via Functional Groups, 2nd ed. John Wiley and Sons, New York 1954.

    Google Scholar 

  • Sjöquist J., Meloun B., Hjelm H.: Protein A isolated fromStaphylococcus aureus after digestion with lysostaphin.Eur J. Biochem.29, 572–578 (1972).

    PubMed  Article  Google Scholar 

  • Tucker D.F., Begent R.H.J., Hogg N.M.: Characterization of immune complexes in serum by adsorption on staphylococcal Protein A: Model studies and application to sera of rats bearing a gross virus induced lymphoma.J. Immunol.121, 1644–1651 (1978).

    PubMed  CAS  Google Scholar 

  • Vogel A.I.:A Textbook of Quantitative Inorganic Analysis, Theory and Practice, 2nd ed. Longmans, Green & Co., New York 1951.

    Google Scholar 

  • Werner S., Machleidt W.: Isolation of precursors of cytochrome oxidase fromNeurospora crassa: Application of subunit-specific antibodies and Protein A fromStaphylococcus aureus.Eur. J. Biochem.90, 99–105 (1978).

    PubMed  Article  CAS  Google Scholar 

  • Zaniewski R., Roy S.: Protein A immobilised affinity cartridge for immunoglobulin purification.Biotechnol. Appl. Biochem.37, 257 (1991).

    Google Scholar 

Download references

Author information

Affiliations

  1. Research and Development, Hindustan Antibiotics Ltd., Pimpri, 411 018, Pune, India

    R. G. Muley, S. Sarkar, S. Ambedkar & S. R. Naik

Authors
  1. R. G. Muley
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Sarkar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Ambedkar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. R. Naik
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Muley, R.G., Sarkar, S., Ambedkar, S. et al. Influence of alkali-treated cornsteep liquor containing medium on protein A production byStaphylococcus aureus . Folia Microbiol 43, 31–34 (1998). https://doi.org/10.1007/BF02815537

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02815537

Keywords

  • Staphylococcus Aureus
  • Extracellular Protein
  • Lymphokine Activate Killer Cell
  • Trace Trace
  • Staphylococcal Protein