Abstract
Removal of Mn2+ by EDTA treatment converted dodecameric glutamine synthetase (GS) fromStreptomyces aureofaciens into inactive subunits but did not affect significantly their conformation. However, when fractionated by gel filtration FPLC, the Mn2+-free subunits showed a 7-fold increase ofA 280, probably due to a significant alteration in their tertiary structure. Mn2+ reduced theA 280 of the subunits and promoted their reaggregation to form active GS. Mg2+ or Ca2+ but not Co2+ or Zn2+ might have similar effects. The results suggest that specific divalent cations might play a crucial role in stabilizing subunit interactions as well as the conformation of the individual subunits inStreptomyces GS. The role of specific divalent cations in the regulation of GS turnover is discussed.
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Nguyen, K.T., Nguyen, L.T., Kopecký, J. et al. Removal of Mn2+ induces dissociation of glutamine synthetase fromStreptomyces aureofaciens . Folia Microbiol 42, 539–543 (1997). https://doi.org/10.1007/BF02815461
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DOI: https://doi.org/10.1007/BF02815461