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Novel mitochondrial proteins and decreased intrinsic activity of cytochrome-C Oxidase

Characteristics of swayback disease in sheep

  • Part XII Clinical and Experimental Investigations in the Caribbean
  • Published:
Molecular and chemical neuropathology

Abstract

Swayback disease (SD), a fatal neurological disorder affecting lambs and kids, is characterized by abnormal mitochondria and low cytochrome-c oxidase activity. The cytochrome-c oxidase content and activity and the protein composition of mitochondria isolated from the brains of SD lambs were investigated. Difference spectra analysis indicated that the cytochrome-c oxidase content of mitochondria from SD animals was lower than normal, and electrophoresis showed that when compared to mitochondria from normal animals, lipid-depleted mitochondria from SD lambs had a different protein composition, particularly, in the 40–55 kDa region. Polarographic studies, using cytochrome-c as substrate, confirmed low intrinsic activity of cytochrome-c oxidase within the mitochondria of SD lambs. These studies also showed that at low ionic strength, such mitochondria did not yield the expected characteristic biphasic Eadie-Hofstee plots.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medical Sciences, The University of the West Indies, Uriah Butler Highway, Mount Hope, St. Augustine, Trinidad and Tobago

    Trevor Alleyne, Andrew Adogwa, Anthony Lalla, Jerome Joseph & Roger John

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  1. Trevor Alleyne
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  2. Andrew Adogwa
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  3. Anthony Lalla
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  4. Jerome Joseph
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  5. Roger John
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Alleyne, T., Adogwa, A., Lalla, A. et al. Novel mitochondrial proteins and decreased intrinsic activity of cytochrome-C Oxidase. Molecular and Chemical Neuropathology 28, 285–293 (1996). https://doi.org/10.1007/BF02815234

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  • DOI: https://doi.org/10.1007/BF02815234

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