Abstract
The effect of lignocellulosic hydrolysate of crushed corn mobs on the growth and lactic acid formation in a continuous culture ofLactobacillus casei andL. lactis at dilution rate 0.08–0.3/h was studied. A simple physiological model of the process was derived from computer-aided analysis of the data which relates bacterial growth, lactic acid formation to the utilization of two different sources of nutrients. The parameters of the model were estimated by nonlinear regression and used for process simulation and estimation of maximum productivity of lactic acid.
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Abbreviations
- D :
-
dilution rate, 1/h
- L:
-
concentration of lactic acid, g/L
- k 1,k 2 k 3 :
-
kinetic parameters, 1/h, 1/h, L g−1 h−1
- K S1 :
-
saturation constant, g/L
- S1, S1 0 :
-
concentration of glucose in the fermentor and in the fresh medium, g/L
- S2, S2 0 :
-
concentration of unknown growth supporting substrate equivalent to dry matter of biomass formed per L, g/L
- RNA, RNAmin :
-
mass fraction of RNA in the growing and nongrowing biomass
- YL/S1 :
-
yield of lactic acid produced based on glucose consumed
- y:
-
dimesionless concentration of RNA defined in Eq. 5
- X:
-
dry matter of biomass, g/L
- μ:
-
specific growth rate, 1/h
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Melzoch, K., Votruba, J., Schwippel, J. et al. Lactic acid production in a continuous culture using lignocellulosic hydrolysate as a substrate. Identification of a physiological model. Folia Microbiol 41, 211–215 (1996). https://doi.org/10.1007/BF02814702
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DOI: https://doi.org/10.1007/BF02814702