Abstract
A number of methionine, methionine+lysine-, and (methionine+lysine+isoleucine)-auxotrophic mutants producing threonine have been isolated from a glutamate-producing strain ofArthrobacter globiformis by a three-step mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine. The best double mutant ML24 requiring methionine and lysine for growth produced 3.2 gl-threonine per L in the synthetic Alföldi medium (200 mmol/L) glucose, 80 mmol/L ammonium nitrate) supplemented with 5 μg biotin per L and optimum (0.5 mmol/L) methionine and lysine concentrations.
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Das, Y.B., Mondal, S. l-threonine production by polyauxotrophic mutants ofArthrobacter globiformis . Folia Microbiol 41, 141–145 (1996). https://doi.org/10.1007/BF02814689
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DOI: https://doi.org/10.1007/BF02814689