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Immobilization of whole cells ofStreptomyces kanamyceticus containing glucose isomerase by a combination of heat treatment in the presence of minerals and entrapment in calcium alginate gels

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Abstract

A method of immobilization of whole cells ofStreptomyces kanamyceticus containing glucose isomerase was devised, based on techniques of heat fixation in the presence of minerals and, entrapment in calcium alginate gels. The optimum activity of the enzyme was obtained when the cells were heat-fixed at 60°C for 10 min in the presence of 50 mmol/L MgSO4·7H2O and 5 mmol/L CoCl2·6H2O and then cast into calcium alginate beads using 2% sodium alginate.

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Authors and Affiliations

  1. Department of Food Technology and Biochemical Engineering, Jadavpur University, 700 032, Calcutta, India

    Sikha Mukhopadhyay

  2. Department of Life Science and Biotechnology, Jadavpur University, 700 032, Calcutta, India

    S. K. Majumdar

Authors
  1. Sikha Mukhopadhyay
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  2. S. K. Majumdar
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Mukhopadhyay, S., Majumdar, S.K. Immobilization of whole cells ofStreptomyces kanamyceticus containing glucose isomerase by a combination of heat treatment in the presence of minerals and entrapment in calcium alginate gels. Folia Microbiol 41, 502–504 (1996). https://doi.org/10.1007/BF02814667

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  • DOI: https://doi.org/10.1007/BF02814667

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