Abstract
The level of proteinase activity and the ratio of proteinases I and II, secreted byAspergillus terreus, a cellulase producer, was followed during its growth on media containing various carbon sources. Correlation was found between the level of proteinase secretion and the rate of change of the cellulase complex spectrum. The extracellular proteolytic system ofA. terreus was presented mainly by proteinase II (metalloproteinase) during cultivation under conditions favoring fast accumulation of low-molar mass cellulases. The results indicate that proteinase II could be responsible for the limited proteolysis of high-molar mass cellulases ofA. terreus into smaller enzymes of the cellulolytic complex, thus changing their substrate specificity.
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Beletskaya O.P., Okunev O.N., Kulaev I.S.: Function and regulation of cellulase biosynthesis and secretion inAspergillus terreus, pp. 61–71 in G.K. Skryabin (Ed.):Problems of Biochemistry and Physiology of Microorganisms. Puschino (Russia) 1985.
Beletskaya O.P., Panin A.L., Kulaev I.S.: Changes in the set of endoglucanases in the fungusAspergillus terreus during growth.Priklad. Biokhim. Mikrobiol. 23, 173–178 (1987).
Bradford M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal. Biochem. 72, 248–254 (1976).
Haab D., Hagspiel K., Szakmary K., Kubicek C.P.: Formation of the extracellular proteases fromTrichoderma reesei QM-9414 involved in cellulase degradation.J. Biotechnol. 16, 187–198 (1990).
Hagspiel K., Haab D., Kubicek C.P.: Protease activity and proteolytic modification of cellulases from aTrichoderma reesei QM 9414 selectant.Appl. Microbiol. Biotechnol. 32, 61–67 (1989).
Knowles J., Lehtovaara P., Teeri T.T.: Cellulase families and their genes.Trends Biotechnol. 5, 255–261 (1987).
Laemmli U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227, 680–685 (1970).
Staikova D., Beletskaya O.P., Kulaev I.S.: Effect of inducers on the set of cellobiases of the fungusAspergillus terreus.Priklad. Biokhim. Mikrobiol. 23, 342–348 (1987).
Stefanova M.E.: Purification of two proteinases fromAspergillus terreu by affinity chromatography.J. Chrom. 639, 346–351 (1993).
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Stefanova, M.E., Beletskaya, O.P. & Kulaev, I.S. Extracellular proteinase spectrum ofAspergillus terreus grown on various substrates. Folia Microbiol 39, 231–234 (1994). https://doi.org/10.1007/BF02814654
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DOI: https://doi.org/10.1007/BF02814654