Abstract
ThelacZ transcriptional fusion assay of the activation of rhizobialnod genes has been adapted for the microtitration plates. The assay can be performed reproducibly on a microscale, including induction, lysis, and spectrophotometry. The technique facilitates processing of a large number of samples in physiological studies. Using the microplate method, the time course of the release ofnod-gene inducers from the root of noninoculated, hydroponically cultivated, pea (Pisum sativum L.) plants was monitored till the 16th day after germination. The released activity increased with plant age but a high variability among individual plants and days was observed.
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Abbreviations
- 2-ME:
-
2-mercaptoethanol
- SDS:
-
sodium dodecyl sulfate
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Novák, K., Škrdleta, V., Němcová, M. et al. Optimization of rhizobialnod gene-inducing activity assay in pea root exudate. Folia Microbiol 39, 208–214 (1994). https://doi.org/10.1007/BF02814650
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DOI: https://doi.org/10.1007/BF02814650