Abstract
The ability ofRhizobium andBradyrhizobium strains to solubilize phosphate from hydroxyapatite was determined in a medium containing NH4Cl or KNO3. The presence of NH4 + in the medium resulted in higher solubilization of phosphate as compared to the presence of KNO3, with the exception ofR. leguminosarium bv. viceae strain TAL 1236 and 1402 which solubilized comparable amounts of phosphate in a medium containing either KNO3 or NH4Cl. These results suggest that the strains employ two different mechanisms for phosphate solubilization, one depending on the presence of NH4 +, the other not requiring its presence. Temperature and aeration (O2 demand) optima were 30°C and 4.2 Hz (shaking frequency), respectively. In nonsterile soil the tested strain (R. meliloti TAL 1236) was very effective in solubilizing rock phosphate.
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References
Asea P.E.A., Kucey R.M.N., Stewart J.W.B.: Inorganic phosphate solubilization by twopenicillium species in solution culture and soil.Soil Biol. Biochem.20, 459–464 (1988).
Banenko YU. S., Tyrygina G.I., Grigor'ev E.F., Dohgikh L.M., Borisow T.I.: Biological activity and physiological-biochemical properties of phosphate dissolving bacteria.Microbiologiya53, 533–539 (1984).
Beever R.E., Burns D.G.W.: Phosphorus uptake, storage and utilization by fungi.Adv. Bot. Res.8, 127–129 (1980).
Chen P.S., Toribara T.Y., Warner H.: Microdetermination of phosphorus.Analyt. Chem.28, 1756–1758 (1956).
Cabala-Rosand P., Wild A.: Direct use of low grade phosphate rock from Brazil as fertilizer. II. Effect of mycorrhizal inoculation and nitrogen source.Plant & Soil65, 363–373 (1982).
Halvorson H.O., Keynan A., Kornberg H.L.: Utilization of calcium phosphates for microbial growth at alkaline pH.Soil Biol. Biochem.22, 887–890 (1990).
Kucey R.M.N.: Phosphate-solubilizing bacteria and fungi in various cultivated and virgin Alberta soils.Can. J. Soil. Sci.63, 671–678 (1983).
Leyval C., Barthelin J.: Interactions betweenLaccaria laccata, Agrobacterium radiobacter and beech roots: Influence on P, K, Mg and Fe mobilization from mineral and plant growth.Plant & Soil117, 103–110 (1989).
Roos W., Luckner M.: Relation between proton extrusion and fluxes of ammonium ions and organic acid inPenicillium cyclopium.J. Gen. Microbiol.130, 1007–1014 (1984).
Thomas G.V.: Occurrence and ability of phosphate-solubilizing fungi from cocconut plant soils.Plant & Soil87, 357–364 (1985).
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Abd-Alla, M.H. Solubilization of rock phosphates byRhizobium andBradyrhizobium . Folia Microbiol 39, 53–56 (1994). https://doi.org/10.1007/BF02814530
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DOI: https://doi.org/10.1007/BF02814530