Skip to main content
SpringerLink
Log in

Characterization of phospholipase A2 of mycoplasma species

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

As phospholipases of mycoplasma species may play a role in the pathogenesis of respiratory tract and urogenital tract diseasesMycoplasma mycoides andAcholeplasma laidlawii were examined as to the production of phospholipase A2 (PLA) and attempts were made to purify and characterize it. Both species produced PLA. The purified enzyme was found to be heat-labile, active at alkaline pH, revealing a single band in polyacrylamide gel electrophoresis. Metal ions such as calcium and barium, increased its activity whereas solvents at high concentrations decreased it. It was resistant to surfactants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ames G.F.: Lipids ofSalmonella typhimurium andEscherichia coli: Structure and metabolism.J. Bacteriol. 95, 833–843 (1968).

    PubMed  CAS  Google Scholar 

  • Bartleit G.R.: Phosphorus assay in column chromatography.J. Biol. Chem. 234, 466–468 (1959).

    Google Scholar 

  • Chanock R.M., Hayflick L., Barile M.F.: Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO.Proc. Nat. Acad. Sci. 48, 41–48 (1962).

    Article  PubMed  CAS  Google Scholar 

  • Cooper T.G.:Electrophoresis, inTools of Biochemistry, p. 194. John Willey and Sons, New York 1977.

    Google Scholar 

  • Desilva N.S., Quinn P.A.: Endogenous activity of phospholipase A and C inUreaplasma urealyticum.J. Clin. Microbiol. 12, 354–358 (1986).

    Google Scholar 

  • Doi O., Nojima S., Ohki M.: Two kinds of phospholipase A lysophospholipase inEscherichia coli.Biochim. Biophys. Acta 260, 249–258 (1972).

    Google Scholar 

  • Embree J.E., Krause V.W., Embil J.A., MacDonald S.: Placental infection withMycoplasma hominis andUreaplasma urealyticum: Clinical correlation.Obstet. Gynecol. 56, 475–481 (1980).

    PubMed  CAS  Google Scholar 

  • Fung C.K., Proulx P.: Metabolism of phosphoglycerides inEscherichia coli. III. Presence of phospholipase A and A2 inEscherichia coli.Canad. J. Biochem. 47, 371–373 (1969).

    Article  CAS  Google Scholar 

  • Gross Z., Rottem S.: The preservation ofMycoplasma carpricolum cell inactness after phospholipase A2 treatment.Biochim. Biophys. Acta 778, 372–378 (1984).

    Article  CAS  Google Scholar 

  • Haberman E., Hardetk L.: A sensitive and specific plate test for the quantification of phospholipases.Ann. Biochem. 50, 163–173 (1972).

    Article  Google Scholar 

  • Ispolatorskaya M.V.: pp. 109–158 inMicrobial Toxins, Vol. II (Ed. S. Kadis, T. C. Montie, Ajil, Montie). Academic Press, New York-London 1971.

    Google Scholar 

  • Kent C., Lennasz W.J.: An osmotically fragile mutant ofBacillus subtilis with an active membrane associated phospolipase A.Proc. Nat. Acad. Sci. 69, 2793–2797 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Lehmann V.: The nature of phospholipase C fromAcinetobacter calcoacelicous. Effects on whole red cells and red cell membranes.Acta Path. Microbiol. Scand. Sect. B 81, 419–421 (1973).

    CAS  Google Scholar 

  • Linkisch P.G., Vogt W.: Direct hemolytic activity of phospholipase A.Biochim. Biophys. Acta 270, 241–247 (1972).

    Google Scholar 

  • Magee W.L., Thompsen R.H.B.: The estimation of phospholipase A activity in aqueous systems.Biochem. J. 77, 526–534 (1960).

    PubMed  CAS  Google Scholar 

  • Magnusson B.J., Docry H.M., Gulasekharam J.: Phospholipase activity of staphylococcal toxin.Nature 196, 270–273 (1962).

    Article  CAS  Google Scholar 

  • Miller G.L.: Use of dinitrosalicylic acid for determination of reducing sugars.Anal. Chem. 31, 426–428 (1959).

    Article  CAS  Google Scholar 

  • Nygren B., Hoborn J., Wahlen P.: Phospholipase A production inStaphylococcus aureus.Acta Path. Microbiol. Scand. 68, 429–433 (1966).

    PubMed  CAS  Google Scholar 

  • Okama Y., Yamaguchi T.: Studies on phospholipase fromStreptomyces purification and properties ofStreptomyces lachiyoenhis phospholipase D.J. Biochem. 78, 363–373 (1975).

    Google Scholar 

  • Ono Y., Nojima S.: Phospholipase of the membrane fraction ofMycobacterium phlei.Biochim. Biophys. Acta 76, 111–119 (1969).

    Google Scholar 

  • Pasten I., Macchina V., Katsen R.: A phospholipase specific for sphingomyelin fromClostridium perfringens.J. Biol. Chem. 243, 3750–3755 (1968).

    Google Scholar 

  • Proulx P.R., Van Deenen L.L.M.: Phospholipase activities ofEscherichia coli.Biochim. Biophys. Acta 144, 171–174 (1967).

    PubMed  CAS  Google Scholar 

  • Quinn P.A., Butamy J., Chimpora M., Taylor J., Mannal W.: Prospective study of microbial infection in still births and early neonatal death.Am. J. Obstet. Gynecol. 151, 238–249 (1985).

    PubMed  CAS  Google Scholar 

  • Randerath K.:Thin Layer Chromatography. Springer Verlag, Berlin 1965.

    Google Scholar 

  • Rao R.H., Subrahmanyam D.: Studies on the phospholipase A in larvae ofCulex pipiens fatigans.J. Insect. Physiol. 15, 149–153 (1961).

    Article  Google Scholar 

  • Raybin D.M., Bertsch L.L., Kornberg A.: A phospholipase inBacillus megaterium unique to spores and sporangia.Biochemistry 11, 1754–1760 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Rottem S., Hasin M., Razin S.: Difference in susceptibility to phospholipase C of free and membrane bound phospholipids ofMycoplasma hominis.Biochim. Biophys. Acta 323, 520–531 (1973).

    Article  PubMed  CAS  Google Scholar 

  • Scherizen J.J., Van Groningenen, Luyben W.A.H.H.: Phospholipase C fromBacillus cereus andClostridium welchi.Biochim. Biophys. Acta 649, 1–12 (1981).

    Article  Google Scholar 

  • Sneff L.M., Wagener W.S., Brooks G.F., Finnerty W.R., Makula K.A.: Phospholipids composition of phospholipase A ofNeisseria gonorrhoeae.J. Bacteriol. 127, 874–880 (1976).

    Google Scholar 

  • Sonoki S., Ikezawa H.: Studies on phospholipase C fromPseudomonas aureofaciens: Purification and some properties of phospholipase C.Biochim. Biophys. Acta 403, 412–424 (1975).

    PubMed  CAS  Google Scholar 

  • Stanier R., Palleroni N.J., Doudoroff M.: The aerobicPseudomonas, a taxonomic study.J. Gen-.Microbiol. 43, 159–271 (1966).

    PubMed  CAS  Google Scholar 

  • Van Golde L.M.C., McElhaney R.N., Van Deenen L.L.M.: A membrane bound lysophospholipase fromMycoplasma laidlawii strain B.Biochim. Biophys. Acta 231, 245–249 (1971).

    PubMed  Google Scholar 

  • Verkleij A.J., Zwall R.F.A., Roelofsen B., Comfurius P., Kastelinjn D., Van Deenen L.L.M.: The asymmetric distribution of phospholipids in human red cell membrane. A combined study using phospholipases and freeze-etching electron microscopy.Biochim. Biophys. Acta 323, 178–193 (1973).

    Article  PubMed  CAS  Google Scholar 

  • Writlind B., Heden L., Sjoberg L., Wadstrom C.: Production of enzymes and toxins by hospital strain ofPseudomonas aeruginosa in relation to serotypes and phage typing pattern.J. Med. Microbiol. 6, 91–100 (1973).

    Article  Google Scholar 

  • Zwaal R.F.A., Roelofsen B., Compfurius P., Van Deenen L.L.M.: Organization of phospholipids in human red cell membranes as detected by the action of various purified phospholipases.Biochim. Biophys. Acta 406, 83–86 (1975).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, Panjab University, 160 014, Chandigarh, India

    S. Bhandari & P. J. Asnani

Authors
  1. S. Bhandari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. J. Asnani
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bhandari, S., Asnani, P.J. Characterization of phospholipase A2 of mycoplasma species. Folia Microbiol 34, 294–301 (1989). https://doi.org/10.1007/BF02814471

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02814471

Keywords

Search

Navigation