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Conversion of disaccharides to 3-ketodisaccharides by nongrowing and immobilized cells ofAgrobacterium tumefaciens

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Abstract

High-performance liquid chromatography was used to estimate 3-ketolactose and 3-ketosucrose in cultures of agrobacteria. The activities of enzymes that convert the disaccharide substrate were evaluated during batch cultivation ofAgrobacterium tumefaciens on sucrose, maltose, and lactose. The highest activity of glucoside 3-dehydrogenase and a slight activity of disaccharide-hydrolyzing enzymes were found in cells grown on lactose. Nongrowing cells converted lactose to 3-ketolactose faster than immobilized cells did. Immobilization of cells into polysaccharide gels did not stabilize the activity of glucoside 3-dehydrogenase. Glutaraldehyde cross-linking of the cell content led to an inactivation of the respiratory chain but Fe3+ could be used as an electron acceptor. Cells treated with glutaraldehyde converted lactose faster than nongrowing ones but the activity of glucoside 3-dehydrogenase was not stable.

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References

  • Bernaerts M.J., De Ley J.: 3-Ketoglycosides, new intermediates in the bacterial catabolism of disaccharides.Biochim. Biophys. Acta30, 666–668 (1958).

    Article  Google Scholar 

  • Bernaerts M.J., De Ley J.: Microbiological formation and preparation of 3-ketoglycosides from disaccharides.J. Gen. Microbiol.22, 129–136 (1960).

    PubMed  CAS  Google Scholar 

  • Chern C.K., Hayano K., Rusaka I., Fukui S.:d-Glucoside: 3-dehydrogenase as a common factor for various transport reactions inAgrobacterium tumefaciens.Agric. Biol. Chem.40, 2193–2197 (1976).

    CAS  Google Scholar 

  • Eltz R.W.: Food antioxidants. US Pat. 3 372 036 (1968).

  • Fukui S.: Transformation of glucose to 3-ketoglucose with the cells ofAgrobacterium tumefaciens.Biochem. Biophys. Res. Commun.18, 186–191 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Fukui S.: Biochemical conversion of uridine diphosphate glucose to uridine diphosphate 3-ketoglucose by washing cells ofAgrobacterium tumefaciens.Agric. Biol. Chem.34, 321–324 (1970).

    CAS  Google Scholar 

  • Fukui S., Hayano K.: Micro methods for determination of 3-ketosucrose and 3-ketoglucose.Agric. Biol. Chem.33, 1013–1017 (1969).

    CAS  Google Scholar 

  • Fukui S., Hochster R.S.: Conversion of disaccharides to the corresponding glycoside-3-uloses by intact cells ofAgrobacterium tumefaciens.Can. J. Biochem. Physiol.41, 2363–2371 (1963).

    PubMed  CAS  Google Scholar 

  • Hayano K., Fukui S.: Purification and properties of 3-ketosucrose-forming enzyme from the cells ofAgrobacterium tumefaciens.J. Biol. Chem.242, 3665–3675 (1967).

    CAS  Google Scholar 

  • Klekner V., Šimová E., Řiĉica J.: Lactose utilization byAgrobacterium. (In Czech)Kvas. prům.32, 286 (1986).

    Google Scholar 

  • Korandová Z.: Production of 3-ketosaccharides byAgrobacterium tumefaciens.MSc Thesis. (In Czech) Institute of Chemical Technology, Prague 1987.

    Google Scholar 

  • Kurowski W.M., Fensom A.H., Pirt S.J.: Factors influencing the formation and stability ofd-glucoside 3-dehydrogenase activity in cultures ofAgrobacterium tumefaciens.J. Gen. Microbiol.90, 191–202 (1986).

    Google Scholar 

  • Lancy E.D., Tuovinen O.H.: Ferrous ion oxidation byThiobacillus ferrooxidans immobilized in calcium alginate.Appl. Microbiol. Biotechnol.20, 94–99 (1984).

    Article  CAS  Google Scholar 

  • Löbl V.: Isolation of 3-ketosaccharides from the cultivation media ofAgrobacterium tumefaciens.MSc Thesis. (In Czech) Institute of Chemical Technology, Prague 1987.

    Google Scholar 

  • Nakamura L.R., Tyler D.D.: Induction ofd-aldohexoside: cytochrome-c oxidoreductase inAgrobacterium tumefaciens.J. Bacteriol.129, 830–835 (1977).

    PubMed  CAS  Google Scholar 

  • Tyler D.D., Nakamura L.K.: Condition for production of 3-ketomaltose fromAgrobacterium tumefaciens.Appl. Microbiol.21, 175–180 (1971).

    Google Scholar 

  • Van Beeumen J., De Ley J.: Hexopyranoside: cytochrome-c oxidoreductase fromAgrobacterium tumefaciens.J. Biochem.6, 331–343 (1968).

    Google Scholar 

  • Van Beeumen J., De Ley J.: Polirography of 3-ketosugars.Bull. Soc. Chim. Belg.80, 683–699 (1971).

    Article  Google Scholar 

  • Wise C.S., Dimler R.J., Davis H.A., Rist C.E.: Determination of easily hydrolyzable fructose units in dextran preparations.Anal. Chem.27, 33–36 (1955).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague 4

    V. Klekner, V. Löbl, E. Šímová & M. Novák

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  1. V. Klekner
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  2. V. Löbl
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  3. E. Šímová
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  4. M. Novák
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Klekner, V., Löbl, V., Šímová, E. et al. Conversion of disaccharides to 3-ketodisaccharides by nongrowing and immobilized cells ofAgrobacterium tumefaciens . Folia Microbiol 34, 286–293 (1989). https://doi.org/10.1007/BF02814470

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  • DOI: https://doi.org/10.1007/BF02814470

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