Abstract
The effect of inorganic phosphate on the biosynthesis of nebramycin factors2, 4 and5′ was studied inStreptomyces tenebrarius strain A (forming2, 4 and5′ in natural ratios) and its mutants B (forming predominantly2), C (forming2 as the only major product) and D (forming predominantly5′). In phosphate-supplemented complex media, the production of2 in A, B and C was reduced by 20–70%, while the yields of5′ remained unchanged in A and decreased by 30–60% in B. The production of4 increased by 50–90% in A and was fully suppressed in B. In D the biosynthesis of the three factors was inhibited completely.
This is a preview of subscription content, log in via an institution to check access.
References
Harangi J., Deák M., Nánási P.: Determination of the major factors of fermentation of the nebramycin complex by high performance liquid chromatography.J. Liquid Chromatogr. 7, 83–93 (1984).
Stark W.M., Hoehn M.M., Knox N.G.: Nebramycin, a new broad-spectrum antibiotic complex. I. Detection and biosynthesis.Antimicrob. Agents Chemother. 1967, 314–323 (1968).
Stark W.M., Knox N.G., Wilgus R., DuBus R. The nebramycin fermentation: Culture and fermentation development, pp. 61–77 inDevelop. Ind. Microbiol. Vol. 17 (L.A. Undekofler, Ed.) American Institute of Biological Sciences, Washington (DC) 1976.
Stark W.M., Wilgus R.M., DuBus R.: Fermentation studies with aminoglycoside-producing microorganisms, pp. 77–89 inDevelop. Ind. Microbiol., Vol. 21 (L.A. Underkofler, M.L. Wulfs, Eds.). Society for Industrial Microbiology, Virginia 1980.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Todorov, T.K., Mikhailova-Ivanova, N. & Uerová, M.B. Regulation of nebramycin biosynthesis by inorganic phosphate. Folia Microbiol 34, 539–541 (1989). https://doi.org/10.1007/BF02814466
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02814466