Abstract
A mixture of heterotrophic bacteria and collection strains ofEscherichia coli andPseudomonas fluorescens were immobilized in calcium alginate or pectate gels. Comparison of respiratory activity, substrate uptake and biosynthetic capacity of immobilized cells showed that both types of carriers permit a prolonged preservation of metabolic activity but the transfer of substances through the gel is faster in the pectate. Morphological changes include some intracellular structures, partial shrinkage of the plasma membrane of immobilized cells, and transformation of a rod-like cell shape to an oval one.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Berger R., Langhammer G.: Immobilization ofCandida lipolytica cells in polyacrylamide gel for citric acid production.Z. Allg. Mikrobiol. 20, 69–72 (1980).
Cassom D., Emery A. N.: On the elimination of artefactual effects in assessing the structure of calcium alginate cell immobilization gels.Enzyme Microb. Technol. 9, 102–106 (1987).
Clarke G.M.:Statistics and Experimental Design. A. Arnold Ltd., London 1969.
Davis B.D., Mingioli E.S.: Mutants ofEscherichia coli requiring methionine or vitamin B 12.J. Bacteriol. 60, 17–28 (1950).
Dawes E. A. Endogenous metabolism and the survival of starved procaryontes, pp. 19–53 in T.G.R. Gray, J.R. Postgate (eds):The Survival of Vegetative Microbes. Cambridge University Press, Cambridge 1976.
Gemeiner P., Kurillová L’., Malovíková, A., Tóth D., Tomašovičová D.: Properties of spherical calcium pectate and alginate gels and their use in diffusion chromatography, solids separations and immobilization of enzymes and cells.Folia Microbiol. 34, 000-000 (1989).
Karelová E., Tóth D., Bricelj M.: Changes in respiratory activity of microorganisms during their incubation in deficient medium. (In Slovak)Biológia (Bratislava) 37, 739–746 (1982).
Kjelleberg S., Harmansson M., Marden P.: The transient phase between growth and nongrowth of heterotrophic bacteria, with emphasis on the marine environment.Ann. Rev. Microbiol. 41, 25–49 (1987).
Koshcheyenko K.A., Turkina M.V., Skriabin G.K.: Immobilization of living microbial cells and their application for steroid transformations.Enzyme Microb. Technol. 5 14–21 (1983).
Krouwel P.G., Braber L.: Ethanol production by yeast at supraoptimal temperatures.Biotechnol. Lett. 1, 403–408 (1979).
Le Chevalier M.W., Hassenauer T.S., Camper A., McFeters G.A.: Disinfection of bacteria attached to granular activated carbon.Appl. Environ. Microbiol. 48, 918–923 (1984).
Mollenhauer H. H.: Plastic embedding mictures for use in electron microscopy.Stain Technol. 39, 111–115 (1964).
Navarro J.M., Durand G.: Modification of yeast metabolism by immobilization onto porous glas.Eur. J. Microbiol. 4, 243–254 (1977).
Radovich J.M.: Mass transfer effects in fermentations using immobilized whole cells.Enzyme Microb. Technol. 7, 1–10 (1985).
Tomašovičová D., Tóth D., Gemeiner P.: Metabolism of bacterial cells immobilized on solid carriers (In Slovak)Biológia (Bratislava) 42, 231–237 (1987).
Tóth D.:The Starving Process and Resulting Metabolic Changes in Escherichia coli. (In Slovak) Veda, Bratislava 1976.
Tóth D., Karelová E.: Interrelation between enzyme activity and ability of multiplying ofEscherichia coli cells after exposure to deficient environment.Biológia (Bratislava) 32, 399–408 (1977).
Venable Y. H., Coggeshall R.: A simplified lead citrate stain for use in electron microscopy.J. Cell Biol. 22, 407–408 (1965).
Woodward J.: Methods of immobilization of microbial cells.J. Microbiol. Methods 8, 91–102 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tóth, D., Tomašovičová, D., Gemeiner, P. et al. Metabolic characteristics of bacterial cells entrapped in beaded calcium alginate and/or pectate gels. Folia Microbiol 34, 515–524 (1989). https://doi.org/10.1007/BF02814463
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02814463