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Water-soaking reaction inhibitory metabolite excreted byXanthomonas campestris pv.malvacearum

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Abstract

The total cultural filtrate (TCF) ofXanthomonas campestris pv.malvacearum (i.e. cultural filtrate after removal of bacterial cells) induced non-persistent watersoaking (WS), on infiltration, in cotton. However diethyl-ether-extracted TCF produced persistent WS reaction. Diethyl-ether-extracted metabolite (EEM) did not induce any reaction but when EEM was co-inoculated with CF (TCF after extraction with diethyl ether), the persistence of WS on cotton was reduced, indicating the presence of an inhibitory metabolite (for WS) in EEM. Analysis of EEM indicated the presence of ammonia, iron and sulfate. Apparently, NH3, Fe and SO4 were covalently linked with diethyl-ether-extracted extracellular matrix. Ferrous/ferric ammonium sulfate, on co-inoculation and/or preinoculation toX. campestris in cotton, was shown to inhibit both susceptible (SR) and hypersensitive (HR) reactions. Inactivation of WS inducing factor by ferric/ferrous ammonium sulfate seem to be the cause for inhibition of induction and/or persistence of SR and HR in cotton.

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References

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Author notes

  1. S. G. Borkar

    Present address: Department of Plant Pathology, J.N.K.V.V, College of Agriculture, 450 001, Khandwa, (M.P), India

Authors and Affiliations

  1. Division of Mycology and Plant Pathology, Indian Agriculture Research Institute, 110 012, New Delhi, India

    S. G. Borkar & J. P. Verma

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  1. S. G. Borkar
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  2. J. P. Verma
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Borkar, S.G., Verma, J.P. Water-soaking reaction inhibitory metabolite excreted byXanthomonas campestris pv.malvacearum . Folia Microbiol 34, 498–503 (1989). https://doi.org/10.1007/BF02814460

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  • DOI: https://doi.org/10.1007/BF02814460

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