Skip to main content
SpringerLink
Log in

Variability in the production of amylase by three isolates ofMyrothecium roridum

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Amylase production by three isolates ofMyrothecium roridum under different cultural conditions was studied. Starch followed by dextrin induced maximum amylase production (dextrinizing and saccharifying) by all the three isolates. Glucose was a poor substrate for the production of amylases. Bitter gourd isolate was a comparatively more efficient producer of amylases than the other two isolates. Addition of glucose to the starch medium resulted in a repression of amylases. Urea was a good source of nitrogen for induction of dextrinizing amylase in bitter gourd and pearl millet isolates.l-Asparagine,l-tyrosine were good sources of nitrogen for induction of saccharifying amylase in bitter gourd, water melon and pearl millet isolate, respectively. With a few exceptions, dextrinizing amylase production was inhibited by gibberellic acid, cycocel, calcium chloride and calcium sulfate, while these substances stimulated saccharifying-amylase production. No correlation could be observed between amylase production and vegetative growth. Amylases of all the three isolates ofM. roridum were characterized.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barfoed H.C.: Central Foods World21, 588 (1976).

    CAS  Google Scholar 

  • Barnett E.A., Fergus C.L.: The relation of extracellular enzymes of mycelium and time in some thermophilic and mesophilicHumicola species.Mycopath. Mycol. Appl. 44, 131 (1971).

    Article  CAS  Google Scholar 

  • Chary S.J., Reddy S.M.: Starch-degrading enzymes of two species ofFusarium.Folia Microbiol. 30, 452 (1985).

    Article  CAS  Google Scholar 

  • Charya M.A.S., Reddy S.M.: Production of amylases by two seed borne fungi.J. Indian Bot. Soc. 62, 147 (1983).

    Google Scholar 

  • Fergus C.L.: The production of amylase by some thermophilic fungus.Mycologia 61, 1171 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Fogarty W.M.:Microbial Enzymes and Biotechnology. Applied Science Publishers 1983.

  • Ghosh A.K., Tandon R.N.: Utilization of polysaccharides by some anthracnose fungi.Proc. Nat. Acad. Sci. (India) 35, 383 (1965).

    Google Scholar 

  • Madsen G.B., Norman B.E., Slott S.:Die Starke 25, 304 (1973).

    Article  CAS  Google Scholar 

  • Manning G.B., Campbell L.L.: Thermostable α-amylade ofBacillus stearothermophillus.J. Biol. Chem. 236, 2952 (1961).

    PubMed  CAS  Google Scholar 

  • Mukherjee S.K., Majumdar S.K.: Fermentation production of amylase byAspergillus flavus.Indian J. Exp. Biol. 11, 436 (1973).

    PubMed  CAS  Google Scholar 

  • Olutiola P.O.: α-Amylolytic activity ofAspergillus chevalieri from mouldy maize seeds.Indian Phytopath. 35, 428 (1982).

    CAS  Google Scholar 

  • Plumer D.T.:An Introduction to Practical Biochemistry. TATA McGraw Hill Publ. Co., New Delhi 1971.

    Google Scholar 

  • Pfueller S.L., Elliot W.H.: The extracellular α-amylase ofBacillus stearothermophilus.J. Biol. Chem. 224, 48 (1969).

    Google Scholar 

  • Reddy S.M.: Utilization of polysaccharides by five species ofHelminthosporium labdev.J. Sci. Technol. 9, 49 (1970).

    Google Scholar 

  • Tayal M.S., Sharma S.M., Agarwal M.L.: Studies on the polyphenols, proteins, chlorophylls, IAA-oxidase and amylases of normal and false smut infected leaves ofPhoenix sylvestris.Indian Phytopath. 34, 337 (1981).

    CAS  Google Scholar 

  • Yabuki M., Ono N., Hoshino K., Fukui S.:Appl. Environ. Microbiol. 34, 1 (1977).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, Kakatiya University, 506 009, Warangal, India

    P. B. Reddy & S. M. Reddy

Authors
  1. P. B. Reddy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. M. Reddy
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Reddy, P.B., Reddy, S.M. Variability in the production of amylase by three isolates ofMyrothecium roridum . Folia Microbiol 34, 471–478 (1989). https://doi.org/10.1007/BF02814456

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02814456

Keywords

Search

Navigation