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D-Amino-acid oxidase—an improved production of the enzyme by the yeastTrigonopsis variabilis in a laboratory fermentor

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Abstract

The cultivation of the yeastTrigonopsis variabilis producingd-amino-acid oxidase (an enzyme participating in the transformation of cephalosporin C into 7-aminocephalosporanic acid for the production of β-lactam antibiotics) was controlled by changes of dissolved oxygen tension and extended fermentation times. The production technology was optimized on a laboratory scale and scale-up parameters were identified.

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Abbreviations

CER:

CO2 evaluation rate. L/min CO2 per L working volume

DAO:

d-amino acid oxidase

DOT:

dissolved oxygen tension, %

μ:

specific growth rate, 1/h

OUR:

oxygen uptake rate, L/min O2 per L working volume

π:

specific production rate, U/h per g dry biomass

RQ:

respiration quotient

X :

dry biomas, g/L

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Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20, Prague, Czechia

    P. Kujan, A. Prell, H. Ŝafář, P. Holler, K. Plháċková & M. Sobotka

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  1. P. Kujan
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  2. A. Prell
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  3. H. Ŝafář
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  4. P. Holler
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  5. K. Plháċková
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  6. M. Sobotka
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Kujan, P., Prell, A., Ŝafář, H. et al. D-Amino-acid oxidase—an improved production of the enzyme by the yeastTrigonopsis variabilis in a laboratory fermentor. Folia Microbiol 46, 427–431 (2001). https://doi.org/10.1007/BF02814434

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  • DOI: https://doi.org/10.1007/BF02814434

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