Abstract
Biochemical aspects of induced autolysis ofSaccharomyces cerevisiae were observed in the presence of various physical and chemical enhancers of autolysis (increased temperature, changes of pH, NaCl, ethanol, fresh autolyzate). Direct assays of proteinases, nucleases, glucanases and acid phosphatases in homogenized autolyzed cells were performed. In addition, the degradation products of proteins, nucleic acids, polysaccharides and phosphate from phosphorylated compounds were determined in the supernatant of autolyzate after centrifugation. These results suggested that the inducers affected transport processes and that they had mostly negative effects on the activities of the above-mentioned enzymes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abel S., Krauss G. J., Glund K.: Single step high performance liquid chromatography analysis of nucleoside monophosphates for ribonuclease activity.J. Chromatogr. 446, 187–189 (1988).
Arnold W.N.:Autolysis. Yeast Cell Envelope—Biochemistry, Biophysics and Ultrastructure, pp. 16–17. Academic Press, New York 1981.
Babayan T.L., Bezrukov M.G., Latov, K.V., Belikov V.M., Belatseva E., Titova E.F.: Induced autolysis ofSaccharomyces cerevisiae: Morphological effects, rheological effects and dynamics of accumulation of extracellular hydrolysis products.Current Microbiol. 5, 163–168 (1981).
Breddam K., Beenfeldt T.: Acceleration of yeast autolysis by chemical methods for production of intracellular enzymes.Appl. Microbiol. Biotechnol. 35, 323–329 (1991).
Chisty Y., Moo-Young M.: Disruption of microbial cells for intracellular products.Enzyme Microb. Technol. 8, 194–204 (1986).
Cornett J.B., Shockmann G.D.: Cellular lysis ofStreptococcus faecalis induced with Triton X-100.J. Bacteriol. 135, 153–160 (1978).
Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F.: Colorimetric methods for determination of sugars and related substances.Anal. Chem. 28, 350–356 (1956).
Dziedzak, J.: Yeasts derivatives.Food Technol. 122–126 (1987).
Keay L., Moser P.W., Wildi B.S.: Proteases of the genusBacillus. II. Alkaline proteases.Biotechnol. Bioeng. 12, 213–219 (1970).
Kollár R., Šturdík E., Farkaš V.: Induction and acceleration of yeast autolysis by addition of fresh autolysate.Biotechnol. Lett. 13, 543–546 (1991).
Low D.: Extraction biochemicals from brewers yeast.Eur. Brew. Conv. 12, 125–129 (1987).
Miller G.L.: Dinitrosalicylic acid reagent for determination of total nucleic acids.Anal. Chem. 31, 426–428 (1956).
Moncolová V., Zemek J.: S-test for determination of glucanases activities.Kvas. Prům. 36, 289–290 (1990).
Peterson G.L.: A simplified method for analysis of inorganic phosphate in the presence of interfering substances.Anal. Biochem. 84, 164–172 (1978).
Spirin A.S.: Qualitative spectrophotometric determination of total nucleic acids.Biokhimiya 23, 656–662 (1958).
Šturdík E., Kollár R., Mikulášová M., Bernát I., Forsthoffer J., Krčmář S.: Fractionation of yeast biomass. II. Optimization of induced autolysis.Kvas. Prům. 34, 241–244 (1988).
Taniguchi M., Tanahashi S., Fuji M.: Properties and repeated use of a reversible-insoluble lytic enzymes.Appl. Microbiol. Biotechnol. 33, 629–635 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kollár, R., Šturdík, E. & Šablatúrová, E. Biochemical, morphological and cytochemical studies of enhanced autolysis ofSaccharomyces cerevisiae . Folia Microbiol 38, 473–478 (1993). https://doi.org/10.1007/BF02814398
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02814398