Conclusion
It must be confessed that the bean extract method outlined above is much more laborious and time-consuming than the procedure utilizing phytohemagglutinin. However, imported products, especially perishable items requiring special conditions for transportation and storage—not only sell at prohibitive prices, but do not always arrive at their destination in the same condition as when they left their country of origin.
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References
Arakaki, D. T., and Sparkes, R. S. (1963). Microtechnique for culturing leucocytes from whole blood.Cytogenetics,2, 57.
Chaudhuri, Amala (1966). Chromosome analysis from whole blood.Indian J. med. Res. 54, 339.
Idem (1967a). Bean extract from,Phaseolus vulgaris in the culture of capillary blood for chromosome analysis.55, 47.
Idem (1967b). Mitogenesis with AB serum.Lancet,2, 1146.
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From the Chaudhuri Centre for Medical Genetics, Calcutta-14.
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Chaudhuri, A. Leucocyte culture with bean extract and AB serum for chromosome analysis. Indian J Pediatr 37, 527–530 (1970). https://doi.org/10.1007/BF02813166
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DOI: https://doi.org/10.1007/BF02813166